Profiling the transcriptome with RNA SPOTs

被引：83

作者：

Eng, Chee-Huat Linus ^{[1
]}

Shah, Sheel ^{[2
]}

Thomassie, Julian ^{[3
]}

Cai, Long ^{[4
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, UCLA Caltech Med Scientist Training Program, Los Angeles, CA 90095 USA

[3] CALTECH, Div Engn & Appl Sci, Pasadena, CA 91125 USA

[4] CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA

来源：

NATURE METHODS | 2017年 / 14卷 / 12期

基金：

美国国家卫生研究院;

关键词：

IN-SITU HYBRIDIZATION; GENE-EXPRESSION; SINGLE CELLS; DNA; FISH;

D O I：

10.1038/nmeth.4500

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule FISH (smFISH) has been the gold standard for quantifying individual transcript abundances. Here, we scale up multiplexed smFISH to the transcriptome level and profile 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This method, called RNA sequential probing of targets (SPOTs), provides an accurate, flexible, and low-cost alternative to sequencing for profiling transcriptomes.

引用

页码：1153 / +

页数：6

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