The effects of microRNA-34a regulating Notch-1/NF-κB signaling pathway on lipopolysaccharide-induced human umbilical vein endothelial cells

BACKGROUND: Notch-1/NF-kappa B signaling plays a key role in the cecal ligation and puncture (CLP)-induced sepsis. This study aims to investigate the intervention effects of microRNA-34a (miR-34a) lentivirus regulating Notch-1/NF-kappa B signaling pathway on lipopolysaccharide (LPS)-induced human umbilical vein endothelial cells (HUVEC). METHODS: HUVEC were divided into four groups as the following: they were infected with negative control lentivirus (NC group) or miR-34a lentivirus (OE group); LPS (1 mu g/mL) was added on the third day on the basis of NC group and OE group for 24 hours (NC+LPS group or OE+LPS group). The levels of TNF-alpha, IL-1 beta, IL-6, and IL-10 in the cell supernatants, and the mRNA and protein expression of Notch-1 and NF-kappa B in the HUVEC were evaluated. RESULTS: After 24 hours, the levels of TNF-alpha, IL-1 beta, IL-6 in the cell supernatants and the protein expression of NF-kappa B from NC+LPS group were significantly higher than those of NC group, but IL-10 level and the protein expression of Notch-1 in NC+LPS group were the opposite. After intervention of miR-34a lentivirus, the cell supernatants TNF-alpha and the protein expression of NF-kappa B in OE+LPS group after 24 hours markedly decreased compared to NC+LPS group. While the cell supernatants IL-1 beta and IL-6 and the mRNA expression of NF-kappa B slightly decreased in OE+LPS group, IL-10 and the mRNA and protein expression of Notch-1 were the opposite. CONCLUSION: miR-34a regulating Notch-1/NF-kappa B signaling pathway can reduce the HUVEC damage caused by LPS stimulation.