Direct visualization of the trimeric structure of the ASIC1a channel, using AFM imaging

被引：33

作者：

Carnally, Stewart M. ^{[1
]}

Dev, Harveer S. ^{[1
]}

Stewart, Andrew P. ^{[1
]}

Barrera, Nelson P. ^{[2
]}

Van Bemmelen, Miguel X. ^{[3
]}

Schild, Laurent ^{[3
]}

Henderson, Robert M. ^{[1
]}

Edwardson, J. Michael ^{[1
]}

机构：

[1] Univ Cambridge, Dept Pharmacol, Cambridge CB2 1PD, England

[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

[3] Univ Lausanne, Dept Pharmacol, CH-1005 Lausanne, Switzerland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 372卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

acid-sensing ion channels; channel assembly; subunit stoichiometry; atomic force microscopy;

D O I：

10.1016/j.bbrc.2008.05.100

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There has been confusion about the subunit stoichiometry of the degenerin family of ion channels. Recently, a crystal structure of acid-sensing ion channel (ASIC) 1a revealed that it assembles as a trimer. Here, we used atomic force microscopy (AFM) to image unprocessed ASIC1 a bound to mica. We detected a mixture of subunit monomers, dimers and trimers. In some cases, triple-subunit clusters were clearly visible, confirming the trimeric structure of the channel, and indicating that the trimer sometimes disaggregated after adhesion to the mica surface. This AFM-based technique will now enable us to determine the subunit arrangement within heteromeric ASICs. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：752 / 755

页数：4

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