Identification of the hemolysis-associated protein 1 as a cross-protective immunogen of Leptospira interrogans by adenovirus-mediated vaccination

被引:95
作者
Branger, C
Sonrier, C
Chatrenet, B
Klonjkowski, B
Ruvoen-Clouet, N
Aubert, A
André-Fontaine, G
Eloit, M
机构
[1] Ecole Natl Vet Nantes, Unite Bacteriol Med & Mol Leptospires, F-44307 Nantes 03, France
[2] INRA, ENVA, UMR Genet Mol & Cellulaire Genet Virale 955, F-94704 Maisons Alfort, France
关键词
D O I
10.1128/IAI.69.11.6831-6838.2001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
New vaccine strategies are needed for the prevention of leptospirosis, a widespread human and animal disease caused by pathogenic leptospires. Our previous work determined that a protein leptospiral extract conferred cross-protection in a gerbil model of leptospirosis. The 31- to 34-kDa protein fraction of Leptospira interrogans serovar autumnalis was shown sufficient for this purpose. In the present study, N-terminal sequencing of a 32-kDa fraction and Southern blotting of genomic DNA with corresponding degenerated oligonucleotide probes identified two of its constituents: hemolysis-associated protein 1 (Hap1) and the outer membrane Leptospira protein 1 (OmpL1). Adenovirus-mediated Hap1 vaccination induces significant protection against a virulent heterologous Leptospira challenge in gerbils, whereas a similar OmpL1 construct failed to protect the animals. These data indicate that Hap1 could be a good candidate for developing a new generation of vaccines able to induce broad protection against leptospirosis disease.
引用
收藏
页码:6831 / 6838
页数:8
相关论文
共 37 条
[1]  
[Anonymous], 1992, VIROLOGY LAB MANUAL
[2]   ISOLATION OF OUTER SHEATH OF LEPTOSPIRA AND ITS IMMUNOGENIC PROPERTIES IN HAMSTERS [J].
AURAN, NE ;
JOHNSON, RC ;
RITZI, DM .
INFECTION AND IMMUNITY, 1972, 5 (06) :968-&
[3]   SCATTERING OF THE RIBOSOMAL-RNA GENES ON THE PHYSICAL MAP OF THE CIRCULAR CHROMOSOME OF LEPTOSPIRA-INTERROGANS SEROVAR ICTEROHAEMORRHAGIAE [J].
BARIL, C ;
HERRMANN, JL ;
RICHAUD, C ;
MARGARITA, D ;
SAINTGIRONS, I .
JOURNAL OF BACTERIOLOGY, 1992, 174 (23) :7566-7571
[4]   SIGNIFICANCE OF LEPTOSPIRAL HEMOLYSIN IN PATHOGENESIS OF LEPTOSPIRA POMONA INFECTIONS [J].
BAUER, DC ;
EAMES, LN ;
FERGUSON, LC ;
SLEIGHT, SD .
JOURNAL OF INFECTIOUS DISEASES, 1961, 108 (02) :229-&
[5]   Estimating the costs associated with endemic diseases of dairy cattle [J].
Bennett, RM ;
Christiansen, K ;
Clifton-Hadley, RS .
JOURNAL OF DAIRY RESEARCH, 1999, 66 (03) :455-459
[6]   Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli [J].
Chartier, C ;
Degryse, E ;
Gantzer, M ;
Dieterle, A ;
Pavirani, A ;
Mehtali, M .
JOURNAL OF VIROLOGY, 1996, 70 (07) :4805-4810
[7]  
ELLINGHAUSEN HC, 1965, AM J VET RES, V26, P45
[8]   CONSTRUCTION OF A DEFECTIVE ADENOVIRUS VECTOR EXPRESSING THE PSEUDORABIES VIRUS GLYCOPROTEIN GP50 AND ITS USE AS A LIVE VACCINE [J].
ELOIT, M ;
GILARDIHEBENSTREIT, P ;
TOMA, B ;
PERRICAUDET, M .
JOURNAL OF GENERAL VIROLOGY, 1990, 71 :2425-2431
[9]  
Faine S., 1999, LEPTOSPIRA LEPTOSPIR
[10]   LEPTOSPIROSIS [J].
FARR, RW .
CLINICAL INFECTIOUS DISEASES, 1995, 21 (01) :1-6