Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi

被引：14

作者：

Goto, M

Ekino, K

Furukawa, K

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 07期

关键词：

D O I：

10.1128/AEM.63.7.2940-2943.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A modified glucoamylase gene (gla4) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol, 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted and replaced with the hygromycin phosphotransferase gene, The modified glaA was successfully expressed and secreted. The modified glucoamylase possessed higher digestibility of raw corn starch and higher stabilities in response to heat and extreme pH.

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页码：2940 / 2943

页数：4

相关论文

共 22 条

[1]

ALESHIN A, 1992, J BIOL CHEM, V267, P19291

[2] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[3] TRANSFORMATION OF THE INDUSTRIAL STRAIN OF ASPERGILLUS-ORYZAE WITH THE HOMOLOGOUS AMDS GENE AS A DOMINANT SELECTABLE MARKER [J].

GOMI, K ;

KITAMOTO, K ;

KUMAGAI, C .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1992, 74 (06) :389-391

[4] ANALYSIS OF THE RAW STARCH-BINDING DOMAIN BY MUTATION OF A GLUCOAMYLASE FROM ASPERGILLUS-AWAMORI VAR. KAWACHI EXPRESSED IN SACCHAROMYCES-CEREVISIAE [J].

GOTO, M ;

SEMIMARU, T ;

FURUKAWA, K ;

HAYASHIDA, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (11) :3926-3930

[5] ROLE OF THE CARBOHYDRATE MOIETY OF A GLUCOMYLASE FROM ASPERGILLUS-AWAMORI VAR. KAWACHI IN THE DIGESTION OF RAW STARCH [J].

GOTO, M ;

KUWANO, E ;

KANLAYAKRIT, W ;

HAYASHIDA, S .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1995, 59 (01) :16-20

[6] STRUCTURAL STUDIES ON THE O-GLYCOSIDICALLY LINKED CARBOHYDRATE CHAINS OF GLUCOAMYLASE G1 FROM ASPERGILLUS-NIGER [J].

GUNNARSSON, A ;

SVENSSON, B ;

NILSSON, B ;

SVENSSON, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 145 (03) :463-467

[7] STRUCTURE OF THE RAW-STARCH-AFFINITY SITE ON THE ASPERGILLUS-AWAMORI VAR KAWACHI GLUCOAMYLASE-I MOLECULE [J].

HAYASHIDA, S ;

NAKAHARA, K ;

KURODA, K ;

MIYATA, T ;

IWANAGA, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (01) :135-141

[8] MOLECULAR-CLONING OF THE GLUCOAMYLASE-I GENE OF ASPERGILLUS-AWAMORI VAR KAWACHI FOR LOCALIZATION OF THE RAW-STARCH-AFFINITY SITE [J].

HAYASHIDA, S ;

KURODA, K ;

OHTA, K ;

KUHARA, S ;

FUKUDA, K ;

SAKAKI, Y .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (04) :923-929

[9] SELECTIVE SUBMERGED PRODUCTIONS OF 3 TYPES OF GLUCOAMYLASES BY A BLACK-KOJI MOLD [J].

HAYASHIDA, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1975, 39 (11) :2093-2099

[10] ISOLATION OF GENOMIC CLONES CONTAINING THE AMDS GENE OF ASPERGILLUS-NIDULANS AND THEIR USE IN THE ANALYSIS OF STRUCTURAL AND REGULATORY MUTATIONS [J].

HYNES, MJ ;

CORRICK, CM ;

KING, JA .

MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (08) :1430-1439