Analysis of biological demulsification process of water-in-oil emulsion by Alcaligenes sp S-XJ-1

被引:79
作者
Wen, Yue [1 ]
Cheng, Hang [1 ]
Lu, Li-Jun [1 ]
Liu, Jia [1 ]
Feng, Yi [1 ]
Guan, Wei [1 ]
Zhou, Qi [1 ]
Huang, Xiang-Feng [1 ]
机构
[1] Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 20092, Peoples R China
基金
中国国家自然科学基金;
关键词
Demulsification; Demulsifying bacteria; Turbiscan stability analyzer; Cell surface hydrophobicity; Interfacial properties; DE-EMULSIFICATION; PETROLEUM EMULSIONS; SURFACTANTS; COPOLYMERS; STABILITY; BACTERIAL;
D O I
10.1016/j.biortech.2010.05.088
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
A demulsifying strain (S-XJ-1) was isolated from petroleum-polluted soil and identified as Alcaligenes sp. It showed emulsion breaking ratio of 81.3% for W/O emulsion within 24 h when the cell concentration was 500 mg/L. Evolution of water droplets during the biological demulsification process was investigated using a Turbiscan stability analyzer and microphotography. Further investigation focused on cell surface hydrophobicity and oil-water interfacial properties. The biological demulsification process began with rapid dispersal of the cells into the oil phase and adsorption onto the oil-water interface. This occurred due to high cell surface hydrophobicity and the presence of amphiphilic compounds in the cell walls. The cells had higher interfacial activity than the emulsifier molecules, and they displaced some of the emulsifier molecules, which effectively reduced the interfacial tension gradient. As a result, the interfacial film strength decreased, the water droplets coalesced and eventually phase separation occurred. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:8315 / 8322
页数:8
相关论文
共 32 条
[1]   INVESTIGATION OF POTENTIAL BIOSURFACTANT PRODUCTION AMONG PHYTOPATHOGENIC CORYNEBACTERIA AND RELATED SOIL MICROBES [J].
AKIT, J ;
COOPER, DG ;
MANNINEN, KI ;
ZAJIC, JE .
CURRENT MICROBIOLOGY, 1981, 6 (03) :145-150
[2]   Demulsification efficiency of some novel styrene/maleic anhydride ester copolymers [J].
Al-Sabagh, A. M. ;
El-Din, M. R. Noor ;
Morsi, R. E. ;
Elsabee, M. Z. .
JOURNAL OF APPLIED POLYMER SCIENCE, 2008, 108 (04) :2301-2311
[3]  
BURON H, 2001, 3 FORM C NEW CONC ST
[4]   Characterization of de-emulsification capabilities of a Micrococcus species [J].
Das, M .
BIORESOURCE TECHNOLOGY, 2001, 79 (01) :15-22
[5]   DEEMULSIFICATION OF PETROLEUM W/O EMULSIONS BY SELECTED BACTERIAL AND YEAST-CELLS [J].
DUVNJAK, Z ;
KOSARIC, N .
BIOTECHNOLOGY LETTERS, 1987, 9 (01) :39-42
[6]   Properties of Langmuir surface and interfacial films built up by asphaltenes and resins:: Influence of chemical demulsifiers [J].
Ese, MH ;
Galet, L ;
Clausse, D ;
Sjöblom, J .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1999, 220 (02) :293-301
[7]   BACTERIA-INDUCED DE-EMULSIFICATION OF OIL-IN-WATER PETROLEUM EMULSIONS [J].
GRAY, NCC ;
STEWART, AL ;
CAIRNS, WL ;
KOSARIC, N .
BIOTECHNOLOGY LETTERS, 1984, 6 (07) :419-424
[8]   Formation and physiological role of biosurfactants produced by hydrocarbon-utilizing microorganisms Biosurfactants in hydrocarbon utilization [J].
Hommel, Rolf K. .
BIODEGRADATION, 1990, 1 (2-3) :107-119
[9]   Characterization and phylogenetic analysis of biodemulsifier-producing bacteria [J].
Huang, Xiang-Feng ;
Guan, Wei ;
Liu, Jia ;
Lu, Li-Jun ;
Xu, Jing-Cheng ;
Zhou, Qi .
BIORESOURCE TECHNOLOGY, 2010, 101 (01) :317-323
[10]   Evaluation of screening methods for demulsifying bacteria and characterization of lipopeptide bio-demulsifier produced by Alcaligenes sp. [J].
Huang, Xiang-Feng ;
Liu, Jia ;
Lu, Li-Jun ;
Wen, Yue ;
Xu, Jing-Cheng ;
Yang, Dian-Hai ;
Zhou, Qi .
BIORESOURCE TECHNOLOGY, 2009, 100 (03) :1358-1365