Utility of separation science in metabolomic studies

被引:43
作者
Issaq, Haleem J. [1 ]
Abbott, Eric [1 ]
Veenstra, Timothy D. [1 ]
机构
[1] SAIC Frederick Inc, Adv Technol Program, Lab Proteom & Analyt Technol, NCI Frederick, Frederick, MD 21702 USA
关键词
chromatography; disease biomarkers; metabolomics; metabonomics; metabolites;
D O I
10.1002/jssc.200700601
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Metabolomics, or alternately metabonomics, an emerging field of biochemical research, is a complementary technique to genomics, transcriptomics, and proteomics. Direct quantitative measurements of metabolite expressions in urine, serum, plasma, and tissue are essential for the study of biological processes in normal and disease states. Since the number of metabolites in a biological sample is large separation science plays an important role in metabolomic research. This review describes the role of GC, HPLC, CE, and supercritical fluid chromatography in metabolomic research. The application of each technique is discussed and its advantages and limitations are discussed with selective illustrative examples.
引用
收藏
页码:1936 / 1947
页数:12
相关论文
共 61 条
[1]   Extraction and GC/MS analysis of the human blood plasma metabolome [J].
A, J ;
Trygg, J ;
Gullberg, J ;
Johansson, AI ;
Jonsson, P ;
Antti, H ;
Marklund, SL ;
Moritz, T .
ANALYTICAL CHEMISTRY, 2005, 77 (24) :8086-8094
[2]   Photoaptamer arrays applied to multiplexed proteomic analysis [J].
Bock, C ;
Coleman, M ;
Collins, B ;
Davis, J ;
Foulds, G ;
Gold, L ;
Greef, C ;
Heil, J ;
Heilig, JS ;
Hicke, B ;
Hurst, MN ;
Husar, GM ;
Miller, D ;
Ostroff, R ;
Petach, H ;
Schneider, D ;
Vant-Hull, B ;
Waugh, S ;
Weiss, A ;
Wilcox, SK ;
Zichi, D .
PROTEOMICS, 2004, 4 (03) :609-618
[3]   Comparison of different search engines using validated MS/MS test datasets [J].
Boutilier, K ;
Ross, M ;
Podtelejnikov, AV ;
Orsi, C ;
Taylor, R ;
Taylor, P ;
Figeys, D .
ANALYTICA CHIMICA ACTA, 2005, 534 (01) :11-20
[4]   Supercritical fluid chromatography-tandem mass spectrometry for fast bioanalysis of R/S-warfarin in human plasma [J].
Coe, Roger A. ;
Rathe, Jonathan O. ;
Lee, Jean W. .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 42 (05) :573-580
[5]   Statistical heterospectroscopy, an approach to the integrated analysis of NMR and UPLC-MS data sets: Application in metabonomic toxicology studies [J].
Crockford, DJ ;
Holmes, E ;
Lindon, JC ;
Plumb, RS ;
Zirah, S ;
Bruce, SJ ;
Rainville, P ;
Stumpf, CL ;
Nicholson, JK .
ANALYTICAL CHEMISTRY, 2006, 78 (02) :363-371
[6]  
DANDENEAU RD, 1979, HIGH RESOLUT CHROMAT, V1, P351
[7]   Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry [J].
De Vos, Ric C. H. ;
Moco, Sofia ;
Lommen, Arjen ;
Keurentjes, Joost J. B. ;
Bino, Raoul J. ;
Hall, Robert D. .
NATURE PROTOCOLS, 2007, 2 (04) :778-791
[8]   Negative mode sheathless capillary electrophoresis electrospray ionization-mass spectrometry for metabolite analysis of prokaryotes [J].
Edwards, JL ;
Chisolm, CN ;
Shackman, JG ;
Kennedy, RT .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1106 (1-2) :80-88
[9]  
Elgstoen KBP, 2001, J CHROMATOGR A, V914, P265
[10]   Capillary electrophoresis for diagnosis of metabolic disease [J].
Elgstoen, KBP ;
Jellum, E .
ELECTROPHORESIS, 1997, 18 (10) :1857-1860