Coupling biomolecules to fullerenes through a molecular adapter

被引:10
作者
Capaccio, M
Gavalas, VG
Meier, MS
Anthony, JE
Bachas, LG [1 ]
机构
[1] Univ Kentucky, Dept Chem, Lexington, KY 40506 USA
[2] Univ Kentucky, Adv Carbon Mat Ctr, Lexington, KY 40506 USA
关键词
D O I
10.1021/bc049861d
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel biotinylated fullerene has been synthesized to facilitate the attachment of biotin-conjugated proteins to C-60 through the use of streptavidin as a molecular adapter. The strong biotin-streptavidin interaction enables the attachment of fullerenes to streptavidin and, because of the availability of four biotin-binding sites on streptavidin, to biotinylated biomolecules. The feasibility of this approach is demonstrated by using biotinylated alkaline phosphatase. Due to the insolubility of fullerenes in aqueous media, the immobilized enzyme can be eventually recovered by simple centrifugation with no significant loss in activity.
引用
收藏
页码:241 / 244
页数:4
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