Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins

被引:185
作者
Choi, Seokheun [1 ]
Goryll, Michael [1 ]
Sin, Lai Yi Mandy [2 ]
Wong, Pak Kin [2 ]
Chae, Junseok [1 ]
机构
[1] Arizona State Univ, Sch Elect Comp & Energy Engn, Tempe, AZ 85287 USA
[2] Univ Arizona, Dept Aerosp & Mech Engn, Tucson, AZ 85721 USA
基金
美国国家科学基金会;
关键词
Microfluidics; Biosensor; Point-of-care detection; Protein; DNA; SURFACE-PLASMON RESONANCE; LABEL-FREE DETECTION; ON-A-CHIP; SINGLE DNA-MOLECULES; NONSPECIFIC-ADSORPTION; RESISTIVE-PULSE; SILICON NANOWIRE; REAL-TIME; ELECTROCHEMICAL BIOSENSORS; NANOPORE ARRAYS;
D O I
10.1007/s10404-010-0638-8
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
This article reviews state-of-the-art microfluidic biosensors of nucleic acids and proteins for point-of-care (POC) diagnostics. Microfluidics is capable of analyzing small sample volumes (10(-9)-10(-18) l) and minimizing costly reagent consumption as well as automating sample preparation and reducing processing time. The merger of microfluidics and advanced biosensor technologies offers new promises for POC diagnostics, including high-throughput analysis, portability and disposability. However, this merger also imposes technological challenges on biosensors, such as high sensitivity and selectivity requirements with sample volumes orders of magnitude smaller than those of conventional practices, false response errors due to non-specific adsorption, and integrability with other necessary modules. There have been many prior review articles on microfluidic-based biosensors, and this review focuses on the recent progress in last 5 years. Herein, we review general technologies of DNA and protein biosensors. Then, recent advances on the coupling of the biosensors to microfluidics are highlighted. Finally, we discuss the key challenges and potential solutions for transforming microfluidic biosensors into POC diagnostic applications.
引用
收藏
页码:231 / 247
页数:17
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