Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope

被引：68

作者：

Ra, Hyejun ^{[1
]}

Piyawattanametha, Wibool ^{[1
,2
,3
,4
]}

Mandella, Michael J. ^{[1
]}

Hsiung, Pei-Lin ^{[3
,4
]}

Hardy, Jonathan ^{[3
,4
]}

Wang, Thomas D. ^{[5
]}

Contag, Christopher H. ^{[3
,4
]}

Kino, Gordon S. ^{[1
]}

Solgaard, Olav ^{[1
]}

机构：

[1] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA

[2] NANOTEC NECTEC, Pathum Thani 12120, Thailand

[3] Stanford Univ, James H Clark Ctr Biomed Engn & Sci, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Pediat Radiol & Microbiol & Immunol, Stanford, CA 94305 USA

[5] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA

来源：

OPTICS EXPRESS | 2008年 / 16卷 / 10期

关键词：

D O I：

10.1364/OE.16.007224

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a handheld dual-axes confocal microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 mu m x 260 mu m. The transverse and axial resolutions of the handheld probe are 1.7 mu m and 5.8 mu m, respectively. Capability to perform real time small animal imaging is demonstrated in vivo in transgenic mice. (C) 2008 Optical Society of America.

引用

页码：7224 / 7232

页数：9

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