Characterization of the Erwinia chrysanthemi gan locus, involved in galactan catabolism

被引:26
作者
Delangle, Aurelie
Prouvost, Anne-France
Cogez, Virginie
Bohin, Jean-Pierre
Lacroix, Jean-Marie
Cotte-Pattat, Nicole Hugouvieux
机构
[1] Univ Sci & Tech Lille Flandres Artois, CNRS, UMR 8576,IFR147, Unite Glycobiol Struct & Fonct, F-59655 Villeneuve Dascq, France
[2] Univ Lyon 1, INSA Lyon, CNRS, Microbiol Adaptat & Pathogenie UMR 5240, F-69622 Villeurbanne, France
关键词
D O I
10.1128/JB.00845-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
beta-1,4-Galactan is a major component of the ramified regions of pectin. Analysis of the genome of the plant pathogenic bacteria Erwinia chrysanthemi revealed the presence of a cluster of eight genes encoding proteins potentially involved in galactan utilization. The predicted transport system would comprise a specific porin GanL and an ABC transporter made of four proteins, GanFGK(2). Degradation of galactans would be catalyzed by the periplasmic 1,4-beta endogalactanase GanA, which released oligogalactans from trimer to hexamer. After their transport through the inner membrane, oligogalactans would be degraded into galactose by the cytoplasmic 1,4-beta-exogalactanase GanB. Mutants affected for the porin or endogalactanase were unable to grow on galactans, but they grew on galactose and on a mixture of galactotriose, galactotetraose, galactopentaose, and galactohexaose. Mutants affected for the periplasmic galactan binding protein, the transporter ATPase, or the exogalactanase were only able to grow on galactose. Thus, the phenotypes of these mutants confirmed the functionality of the gan locus in transport and catabolism of galactans. These mutations did not affect the virulence of E. chrysanthemi on chicory leaves, potato tubers, or Saintpaulia ionantha, suggesting an accessory role of galactan utilization in the bacterial pathogeny.
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页码:7053 / 7061
页数:9
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