Catfish hepcidin gene is expressed in a wide range of tissues and exhibits tissue-specific upregulation after bacterial infection

被引:157
作者
Bao, BL
Peatman, E
Li, P
He, CB
Liu, ZJ
机构
[1] Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA
[2] Auburn Univ, Program Cell & Mol Biosci, Aquat Gen Unit, Auburn, AL 36849 USA
[3] Shanghai Fisheries Univ, EISU, Aquaculture Div, Shanghai 200090, Peoples R China
[4] Shanghai Fisheries Univ, Coll Aquat Life Sci & Technol, Shanghai 200090, Peoples R China
[5] Liaoning Ocean & Fisheries Sci Res Inst, Dalian 116023, Peoples R China
关键词
antimicrobial peptide; hepcidin; gene expression; infection; fish; catfish; Edwardsiella; disease; innate immunity;
D O I
10.1016/j.dci.2005.03.006
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. The cysteine-rich AMPs such as defensin and hepcidin have been extensively studied from various organisms, but their role in disease defense in catfish is unknown. As a first step, we sequenced a hepcidin cDNA from both channel catfish and blue catfish, and characterized the channel catfish hepcidin gene. The channel catfish hepcidin gene consists of two introns and three exons that encode a peptide of 96 amino acids. The amino acid sequences and gene organization were conserved between catfish and other organisms. In contrast to its almost exclusive expression in the liver in humans, the channel catfish hepcidin gene was expressed in a wide range of tissues except brain. Its expression was detected early during embryonic and larval development, and induced after bacterial infection with Edwardsiella ictaluri, the causative agent of enteric septicemia of catfish (ESC) in a tissue-specific manner. The upregulation was observed in the spleen and head kidney, but not in the liver. The expression of hepcidin was upregulated 1-3 days after challenge, but returned to normal levels at 7 days after challenge. The expression profile of the catfish hepcidin gene during the course of bacterial infection mirrors those of inflammatory proteins such as chemokines, suggesting an important role for hepcidin during inflammatory responses. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:939 / 950
页数:12
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