Optimized DNA targeting using N,N-bis(2-pyridylmethyl)-β-alanyl 2′-amino-LNA

被引：33

作者：

Babu, BR ^{[1
]}

Hrdlicka, PJ ^{[1
]}

McKenzie, CJ ^{[1
]}

Wengel, J ^{[1
]}

机构：

[1] Univ So Denmark, Nucl Acid Ctr, Dept Chem, DK-5230 Odense, Denmark

来源：

CHEMICAL COMMUNICATIONS | 2005年 / 13期

关键词：

D O I：

10.1039/b417101b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Incorporation of N,N-bis(2- pyridylmethyl)-beta- alanyl 2'-amino-LNA (bipyridyl-functionalized 2'-amino locked nucleic acid) monomers into DNA strands enables high-affinity targeting of complementary DNA with excellent Watson - Crick selectivity in the presence of divalent metal ions. Positioning of bipyridyl-functionalized 2'-amino-LNA monomers in two complementary DNA strands in a "3'-end zipper'' constitution allows modulation of duplex stability, i.e., a strong stabilizing effect with one equivalent of divalent metal ion per bipyridyl pair, or a strong destabilizing effect with an excess of divalent metal ions.

引用

页码：1705 / 1707

页数：3

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