Conformationally controlled high-affinity targeting of RNA or DNA by novel 2′-amino-DNA/LNA mixmers and pyrenyl-functionalized 2′-amino-DNA

被引：34

作者：

Kalra, N

Babu, BR

Parmar, VS

Wengel, J ^{[1
]}

机构：

[1] Univ So Denmark, Nucleic Acid Ctr, Dept Chem, DK-5230 Odense M, Denmark

[2] Univ Delhi, Dept Chem, Bioorgan Lab, Delhi 110007, India

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2004年 / 2卷 / 20期

关键词：

D O I：

10.1039/b411626g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

9-Mer DNA sequences containing 2'-N-methyl-2'-N-(pyren-1-ylmethyl)-2'-amino-DNA monomers display significantly increased affinity towards DNA complements whereas the corresponding 2'-amino-DNA monomer has a detrimental effect on duplex stability. These effects are efficiently reversed by incorporation of four LNA nucleotides inducing a B-DNA to A-DNA conformational change.

引用

页码：2885 / 2887

页数：3

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