Genome-wide evaluation and discovery of vertebrate A-to-I RNA editing sites

被引：26

作者：

Maas, S. ^{[1
]}

Sie, C. P. Godfried ^{[1
]}

Stoev, I. ^{[2
]}

Dupuis, D. E. ^{[1
]}

Latona, J. ^{[1
]}

Porman, A. M. ^{[1
]}

Evans, B. ^{[1
]}

Rekawek, P. ^{[1
]}

Kluempers, V. ^{[1
]}

Mutter, M. ^{[1
]}

Gommans, W. M. ^{[1
]}

Lopresti, D. ^{[2
]}

机构：

[1] Lehigh Univ, Dept Biol Sci, Bethlehem, PA 18015 USA

[2] Lehigh Univ, Dept Comp Sci & Engn, Bethlehem, PA 18015 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 412卷 / 03期

关键词：

RNA editing; ADAR; Inosine; Adenosine deamination; DATABASE; IDENTIFICATION; ADENOSINE; TARGETS;

D O I：

10.1016/j.bbrc.2011.07.075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA editing by adenosine deamination, catalyzed by adenosine deaminases acting on RNA (ADAR), is a post-transcriptional modification that contributes to transcriptome and proteome diversity and is widespread in mammals. Here we administer a bioinformatics search strategy to the human and mouse genomes to explore the landscape of A-to-I RNA editing. In both organisms we find evidence for high excess of A/G-type discrepancies (inosine appears as a guanosine in cloned cDNA) at non-polymorphic, non-synonymous codon sites over other types of discrepancies, suggesting the existence of several thousand recoding editing sites in the human and mouse genomes. We experimentally validate recoding-type A-to-I RNA editing in a number of human genes with high scoring positions including the coatomer protein complex subunit alpha (COPA) as well as cyclin dependent kinase CDK13. Published by Elsevier Inc.

引用

页码：407 / 412

页数：6

共 18 条

[1] Widespread A-to-I RNA editing of alu-containing mRNAs in the human transcriptome [J].