Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display

被引：39

作者：

Mullaney, BP

Pallavicini, MG

Marks, JD

机构：

[1] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Dept Radiat Oncol, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Dept Anesthesia & Pharmaceut Chem, San Francisco, CA 94143 USA

[4] Univ Calif San Francisco, Ctr Canc, San Francisco, CA 94143 USA

来源：

INFECTION AND IMMUNITY | 2001年 / 69卷 / 10期

关键词：

D O I：

10.1128/IAI.69.10.6511-6514.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25).

引用

页码：6511 / 6514

页数：4

共 28 条

[11]

Jacobsson K, 1996, BIOTECHNIQUES, V20, P1070

[12] COVALENT STRUCTURE OF BOTULINUM NEUROTOXIN TYPE-A - LOCATION OF SULFHYDRYL-GROUPS, AND DISULFIDE BRIDGES AND IDENTIFICATION OF C-TERMINI OF LIGHT AND HEAVY-CHAINS [J].