A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity

被引：251

作者：

Tse, WC

Boger, DL

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2004年 / 37卷 / 01期

关键词：

D O I：

10.1021/ar030113y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.

引用

收藏

页码：61 / 69

页数：9

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