DNA display for in vitro selection of diverse peptide libraries -: art. no. e118

被引：70

作者：

Yonezawa, M

Doi, N

Kawahashi, Y

Higashinakagawa, T

Yanagawa, H ^{[1
]}

机构：

[1] Keio Univ, Dept Biosci & Informat, Yokohama, Kanagawa 2238522, Japan

[2] Waseda Univ, Dept Biol, Sch Educ, Tokyo 1698050, Japan

[3] Ikedarika Sci Corp, Tsukuba Tech Ctr, Tsukuba, Ibaraki 3050062, Japan

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 19期

关键词：

D O I：

10.1093/nar/gng119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the use of a DNA display system for in vitro selection of peptide ligands from a large library of peptides displayed on their encoding DNAs. The method permits completely in vitro construction of a DNA-tagged peptide library by using a wheat germ in vitro transcription/translation system compartmentalized in water-in-oil emulsions. Starting with a library of 10(9)-10(10) random decapeptides, 21 different peptide ligands were isolated for monoclonal antibody anti-FLAG M2. DNA display selected more diverse peptides with a DYKXXD consensus motif than previously reported phage display systems. Binding and recovery rates of three peptides were significantly higher than those of the original FLAG peptide, implying that these peptides would be superior to the FLAG peptide for purification of tagged proteins. The simplicity of DNA display enables two selection rounds per day to be conducted. Further, DNA display can overcome the limitations of previous display technologies by avoiding the use of bacterial cells and RNA tags. Thus, DNA display is expected to be useful for rapid screening of a wide variety of peptide ligands for corresponding receptors.

引用

页数：5

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