Virulence systems of Pseudomonas syringae pv. tomato promote bacterial speck disease in tomato by targeting the jasmonate signaling pathway

被引:256
作者
Zhao, YF
Thilmony, R
Bender, CL
Schaller, A
He, SY
Howe, GA [1 ]
机构
[1] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA
[2] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA
[3] Univ Hohenheim, Inst Plant Physiol & Biotechnol, D-70593 Stuttgart, Germany
[4] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA
[5] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA
关键词
jasmonic acid; Pseudomonas syringae; coronatine; type III secretion; bacterial speck disease; salicylic acid;
D O I
10.1046/j.1365-313X.2003.01895.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000) causes bacterial speck disease on tomato. The pathogenicity of Pst DC3000 depends on both the type III secretion system that delivers virulence effector proteins into host cells and the phytotoxin coronatine (COR), which is thought to mimic the action of the plant hormone jasmonic acid (JA). We found that a JA-insensitive mutant (jai1) of tomato was unresponsive to COR and highly resistant to Pst DC3000, whereas host genotypes that are defective in JA biosynthesis were as susceptible to Pst DC3000 as wild-type (WT) plants. Treatment of WT plants with exogenous methyl-JA (MeJA) complemented the virulence defect of a bacterial mutant deficient in COR production, but not a mutant defective in the type III secretion system. Analysis of host gene expression using cDNA microarrays revealed that COR works through Jai1 to induce the massive expression of JA and wound response genes that have been implicated in defense against herbivores. Concomitant with the induction of JA and wound response genes, the type III secretion system and COR repressed the expression of pathogenesis-related (PR) genes in Pst DC3000-infected WT plants. Resistance of jai1 plants to Pst DC3000 was correlated with a high level of PR gene expression and reduced expression of JA/wound response genes. These results indicate that COR promotes bacterial virulence by activating the host's JA signaling pathway, and further suggest that the type III secretion system might also modify host defense by targeting the JA signaling pathway in susceptible tomato plants.
引用
收藏
页码:485 / 499
页数:15
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