Live-Cell Imaging of Cellular Proteins by a Strain-Promoted Azide-Alkyne Cycloaddition

被引：132

作者：

Beatty, Kimberly E. ^{[1
]}

Fisk, John D. ^{[1
]}

Smart, Brian P. ^{[2
,3
,4
]}

Lu, Ying Ying ^{[1
]}

Szychowski, Janek ^{[1
]}

Hangauer, Matthew J. ^{[2
,3
,4
]}

Baskin, Jeremy M. ^{[2
,3
,4
]}

Bertozzi, Carolyn R. ^{[2
,3
,4
]}

Tirrell, David A. ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 15期

关键词：

alkynes; azides; cycloaddition; fluorescent probes; microscopy; FREE CLICK CHEMISTRY; NEWLY SYNTHESIZED PROTEINS; NONNATURAL AMINO-ACIDS; IN-VIVO INCORPORATION; ESCHERICHIA-COLI; MAMMALIAN-CELLS; GENETIC-CODE; COPPER-FREE; STAUDINGER LIGATION; METHIONINE ANALOGS;

D O I：

10.1002/cbic.201000419

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

[No abstract available]

引用

页码：2092 / 2095

页数：4

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