Recombination proficiency influences frequency and locus of mutational resistance to linezolid in Enterococcus faecalis

被引:56
作者
Lobritz, M
Hutton-Thomas, R
Marshall, S
Rice, LB
机构
[1] Case Western Reserve Univ, Sch Med, Dept Mol Biol & Microbiol, Cleveland, OH 44106 USA
[2] Dept Vet Affairs Med Ctr, Div Infect Dis, Res Serv, Cleveland, OH USA
[3] Dept Vet Affairs Med Ctr, Div Infect Dis, Med Serv, Cleveland, OH USA
关键词
D O I
10.1128/AAC.47.10.3318-3320.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In vitro linezolid resistance was selected at a higher frequency in Enterococcus faecalis JH2-2 than in recombination-deficient E. faecalis UV202. Resistance in JH2-2 was related to accumulated G2576T mutations in 23S rRNA genes, with the least resistance conferred by mutations in two of four copies. UV202 resistance was associated with a G2505A mutation present in a single copy in mutants with different MICs.
引用
收藏
页码:3318 / 3320
页数:3
相关论文
共 15 条
[1]   Evernimicin (SCH27899) inhibits a novel ribosome target site: Analysis of 23S ribosomal DNA mutants [J].
Adrian, PV ;
Mendrick, C ;
Loebenberg, D ;
McNicholas, P ;
Shaw, KJ ;
Klugman, KP ;
Hare, RS ;
Black, TA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (11) :3101-3106
[2]   Oxazolidinone antibiotics target the P site on Escherichia coli ribosomes [J].
Aoki, H ;
Ke, LZ ;
Poppe, SM ;
Poel, TJ ;
Weaver, EA ;
Gadwood, RC ;
Thomas, RC ;
Shinabarger, DL ;
Ganoza, MC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (04) :1080-1085
[3]   Genetic linkage and cotransfer of a novel, vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate [J].
Carias, LL ;
Rudin, SD ;
Donskey, CJ ;
Rice, LB .
JOURNAL OF BACTERIOLOGY, 1998, 180 (17) :4426-4434
[4]   Infections due to vancomycin-resistant Enterococcus faecium resistant to linezolid [J].
Gonzales, RD ;
Schreckenberger, PC ;
Graham, MB ;
Kelkar, S ;
DenBesten, K ;
Quinn, JP .
LANCET, 2001, 357 (9263) :1179-1179
[5]   CONJUGAL TRANSFER OF PLASMID-BORNE MULTIPLE ANTIBIOTIC-RESISTANCE IN STREPTOCOCCUS-FAECALIS VAR ZYMOGENES [J].
JACOB, AE ;
HOBBS, SJ .
JOURNAL OF BACTERIOLOGY, 1974, 117 (02) :360-372
[6]   Resistance mutations in 23 S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center [J].
Kloss, P ;
Xiong, LQ ;
Shinabarger, DL ;
Mankin, AS .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 294 (01) :93-101
[7]   Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis [J].
Marshall, SH ;
Donskey, CJ ;
Hutton-Thomas, R ;
Salata, RA ;
Rice, LB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (10) :3334-3336
[8]   Selection of Enterococcus faecium strains with stable and unstable resistance to the streptogramin RP 59500 using stepwise in vitro exposure [J].
Millichap, J ;
Ristow, TA ;
Noskin, GA ;
Peterson, LR .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1996, 25 (01) :15-20
[9]  
*NAT COMM CLIN LAB, 1997, M7A4 NAT COMM CLIN L
[10]   Risk factors associated with the development of infection with linezolid- and vancomycin-resistant Enterococcus faecium [J].
Pai, MP ;
Rodvold, KA ;
Schreckenberger, PC ;
Gonzales, RD ;
Petrolatti, JM ;
Quinn, JP .
CLINICAL INFECTIOUS DISEASES, 2002, 35 (10) :1269-1272