Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion

被引：40

作者：

Nakano, M ^{[1
]}

Nakai, N ^{[1
]}

Kurita, H ^{[1
]}

Komatsu, J ^{[1
]}

Takashima, K ^{[1
]}

Katsura, S ^{[1
]}

Mizuno, A ^{[1
]}

机构：

[1] Toyohashi Univ Technol, Dept Ecol Engn, Toyohashi, Aichi 4418580, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2005年 / 99卷 / 03期

基金：

日本学术振兴会;

关键词：

reverse transcription polymerase chain reaction; water-in-oil emulsion; single-molecule detection;

D O I：

10.1263/jbb.99.293

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.

引用

页码：293 / 295

页数：3

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