Antigenic stimulation specifically reactivates the replication of archived simian immunodeficiency virus genomes in chronically infected macaques

被引:2
作者
Renoux, C
Wain-Hobson, S
Hurtrel, B
Cheynier, R
机构
[1] Inst Pasteur, Unite Virus Lents, F-75724 Paris, France
[2] Inst Pasteur, Unite Retrovirol Mol, F-75724 Paris, France
[3] Inst Pasteur, Unite Rech & Expertise Physiopathol Infect Lentiv, F-75724 Paris, France
关键词
D O I
10.1128/JVI.79.17.11231-11238.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human immunodeficiency virus/simian immunodeficiency virus (SIV) diversification is a direct consequence of viral replication and occurs principally in secondary lymphoid organs where CD4(+) T cells are activated and proliferate. However, the evolution of viral quasispecies may also be driven by various nonexclusive mechanisms, including adaptation to specific immune responses and modification of viral fitness. Analysis of viral quasispecies in SIV-infected macaques subjected to repeated antigenic stimulations allowed us to demonstrate transient expansions of SIV populations that were highly dependent upon activation of antigen-specific T cells. T-cell clones expanded in response to a particular antigen were infected by a specific viral population and persisted for prolonged periods. Upon a second stimulation by encounter with the same antigen, these specific genomes were at the origin of a new burst of replication, leading to rapid but transient replacement of the viral quasispecies in blood. Finally, longitudinal analysis of SIV sequence variation during and between antigenic stimulations revealed that viral evolution is mostly constrained to periods of strong immunological activity.
引用
收藏
页码:11231 / 11238
页数:8
相关论文
共 52 条
[1]   The infiltration kinetics of simian immunodeficiency virus-specific T cells drawn to sites of high antigenic stimulation determines local in vivo viral escape [J].
Blancou, P ;
Chenciner, N ;
Cumont, MC ;
Wain-Hobson, S ;
Hurtrel, B ;
Cheynier, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (23) :13237-13242
[2]  
Borroto R J, 1997, Rev Panam Salud Publica, V1, P3
[3]   Analysis of HIV-1 env gene sequences reveals evidence for a low effective number in the viral population [J].
Brown, AJL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (05) :1862-1865
[4]   In situ hybridization and immunolabelling study of the early replication of simian immunodeficiency virus (SIVmacJ5) in vivo [J].
Cantó-Nogués, C ;
Jones, S ;
Sangster, R ;
Silvera, P ;
Hull, R ;
Cook, R ;
Hall, G ;
Walker, B ;
Stott, EJ ;
Hockley, D ;
Almond, N .
JOURNAL OF GENERAL VIROLOGY, 2001, 82 :2225-2234
[5]   HIV AND T-CELL EXPANSION IN SPLENIC WHITE PULPS IS ACCOMPANIED BY INFILTRATION OF HIV-SPECIFIC CYTOTOXIC T-LYMPHOCYTES [J].
CHEYNIER, R ;
HENRICHWARK, S ;
HADIDA, F ;
PELLETIER, E ;
OKSENHENDLER, E ;
AUTRAN, B ;
WAINHOBSON, S .
CELL, 1994, 78 (03) :373-387
[6]   Antigenic stimulation by BCG vaccine as an in vivo driving force for SIV replication and dissemination [J].
Cheynier, R ;
Gratton, S ;
Halloran, M ;
Stahmer, I ;
Letvin, NL ;
Wain-Hobson, S .
NATURE MEDICINE, 1998, 4 (04) :421-427
[7]   Insertion/deletion frequencies match those of point mutations in the hypervariable regions of the simian immunodeficiency virus surface envelope gene [J].
Cheynier, R ;
Kils-Hütten, L ;
Meyerhans, A ;
Wain-Hobson, S .
JOURNAL OF GENERAL VIROLOGY, 2001, 82 :1613-1619
[8]   HIV POPULATION-DYNAMICS IN-VIVO - IMPLICATIONS FOR GENETIC-VARIATION, PATHOGENESIS, AND THERAPY [J].
COFFIN, JM .
SCIENCE, 1995, 267 (5197) :483-489
[9]  
Collins Kalonji R., 2002, AIDS Reviews, V4, P165
[10]  
Collins KR, 2000, J ACQ IMMUN DEF SYND, V24, P408, DOI 10.1097/00126334-200008150-00002