A novel biosensor based on a gold nanoflowers/hemoglobin/carbon nanotubes modified electrode

被引：20

作者：

Gao, Ying-Chun ^{[1
]}

Xi, Kai ^{[1
]}

Wang, Wei-Na ^{[1
]}

Jia, Xu-Dong ^{[1
]}

Zhu, Jun-Jie ^{[1
]}

机构：

[1] Nanjing Univ, Nanjing Natl Lab Microstruct, Sch Chem & Chem Engn, State Key Lab Coordinat Chem, Nanjing 210093, Peoples R China

来源：

ANALYTICAL METHODS | 2011年 / 3卷 / 10期

基金：

中国国家自然科学基金;

关键词：

CARBON-PASTE ELECTRODE; DIRECT ELECTROCHEMISTRY; HORSERADISH-PEROXIDASE; HYDROGEN-PEROXIDE; IONIC LIQUID; HEMOGLOBIN; ELECTROCATALYSIS; FILM; BIOELECTROCHEMISTRY; IMMOBILIZATION;

D O I：

10.1039/c1ay05378g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Well-oriented 3D gold flower-like nanoparticles were successfully synthesized by a facile one-pot method, and the gold nanoflowers (AuNFs) were mixed with hemoglobin (Hb) to form a gold nanoflowers/hemoglobin composite. The composite was further combined with multiwalled carbon nanotubes on a glassy carbon electrode (GCE) to fabricate a novel biosensor. The sensor has high stability and bioactivity, and was studied by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The hemoglobin/gold nanoflowers/multiwalled carbon nanotubes glassy carbon electrode (Hb/AuNFs/CNTs/GCE) either retained the Hb in similar native conformations or promoted direct electron transfer. Moreover, the sensor exhibited remarkable catalytic activity toward H(2)O(2) and trichloroacetic acid (TCA). The linear relationship for the determination is in the range of 1.0-60 mu M for H(2)O(2) and 0.06-28 mM for TCA. The detection limits were 0.08 mu M and 7.3 mu M (S/N = 3), respectively.

引用

页码：2387 / 2391

页数：5

共 27 条

[1] DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS [J].

ARMSTRONG, FA ;

HILL, HAO ;

WALTON, NJ .

ACCOUNTS OF CHEMICAL RESEARCH, 1988, 21 (11) :407-413

[2] Recent developments in faradaic bioelectrochemistry [J].

Armstrong, FA ;

Wilson, GS .

ELECTROCHIMICA ACTA, 2000, 45 (15-16) :2623-2645

[3] Carbon nanotubes contain metal impurities which are responsible for the "electrocatalysis" seen at some nanotube-modified electrodes [J].

Banks, CE ;

Crossley, A ;

Salter, C ;

Wilkins, SJ ;

Compton, RG .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (16) :2533-2537

[4] Direct electron transfer and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix [J].

Dai, ZH ;

Liu, SQ ;

Ju, HX ;

Chen, HY .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (08) :861-867

[5]

Gaspar S, 2001, ELECTROANAL, V13, P284, DOI 10.1002/1521-4109(200103)13:4<284::AID-ELAN284>3.0.CO

[6]

2-Z

[7] Direct Electrochemistry and Electrocatalysis of Hemoglobin in Nafion/Carbon Nanochip Film on Glassy Carbon Electrode [J].

George, Sini ;

Lee, Hian Kee .

JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (47) :15445-15454

[8] Amperometric biosensor for hydrogen peroxide and nitrite based on hemoglobin immobilized on one-dimensional gold nanoparticle [J].

Hong, Jianmin ;

Dai, Zhihui .

SENSORS AND ACTUATORS B-CHEMICAL, 2009, 140 (01) :222-226

[9]

HUMACHI I, 1997, J AM CHEM SOC, V119, P9096

[10] ROTATING-RING-DISK ENZYME ELECTRODE FOR BIOCATALYSIS KINETIC-STUDIES AND CHARACTERIZATION OF THE IMMOBILIZED ENZYME LAYER [J].

KAMIN, RA ;

WILSON, GS .

ANALYTICAL CHEMISTRY, 1980, 52 (08) :1198-1205

← 1 2 3 →