Antiviral activity of lycorine against Zika virus in vivo and in vitro

被引：66

作者：

Chen, Huini ^{[1
]}

Lao, Zizhao ^{[2
]}

Xu, Jiangtao ^{[3
]}

Li, Zhaoxin ^{[3
]}

Long, Haishan ^{[3
]}

Li, Detang ^{[4
]}

Lin, Luping ^{[5
]}

Liu, Xiaohong ^{[6
]}

Yu, Liangwen ^{[7
]}

Liu, Weiyong ^{[8
]}

Li, Geng ^{[1
,3
]}

Wu, Jianguo ^{[1
]}

机构：

[1] Jinan Univ, Inst Med Microbiol, Guangdong Key Lab Virol, Guangzhou 510632, Peoples R China

[2] Guangzhou Univ Chinese Med, Math Engn Acad Chinese Med, Guangzhou 510006, Peoples R China

[3] Guangzhou Univ Chinese Med, Lab Anim Ctr, Guangzhou 510006, Peoples R China

[4] Guangzhou Univ Chinese Med, Dept Pharm, Affiliated Hosp 1, Guangzhou 510405, Peoples R China

[5] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Guangzhou 510060, Peoples R China

[6] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510000, Peoples R China

[7] Guangzhou Univ Chinese Med, Guangzhou 510006, Peoples R China

[8] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Lab Med, Wuhan, Peoples R China

来源：

VIROLOGY | 2020年 / 546卷

关键词：

ZIKV; Lycorine; Alkaloid; Antiviral; AG6; mice; RdRp inhibitor; FDA-APPROVED DRUG; INFECTION; IDENTIFICATION; AMARYLLIDACEAE; SOFOSBUVIR; RIBAVIRIN; EFFICACY; BIOLOGY;

D O I：

10.1016/j.virol.2020.04.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

The emergence and re-emergence of Zika virus (ZIKV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas worldwide. Despite these burdens, antiviral therapies do not exist, and inhibitors of ZIKV are therefore urgently needed. To elucidate the anti-ZIKV effect of lycorine, we used reverse transcription-quantitative real-time PCR (qRT-PCR), immunofluorescence, (westernwestern) blot, and plaque forming assay to analyse viral RNA (vRNA), viral protein, progeny virus counts, and validated inhibitors in vitro using a variety of cell lines. Additionally, we found that lycorine acts post-infection according to time-of-addition assay, and inhibits RdRp activity. Lycorine protected AG6 mice against ZIKV-induced lethally by decreasing the viral load in the blood. Due to its potency and ability to target ZIKV infection in vivo and in vitro, lycorine might offer promising therapeutic possibilities for combatting ZIKV infections in the future.

引用

页码：88 / 97

页数：10

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