Stereoselective analysis of benazepril and its stereoisomers by reversed-phase high-performance liquid chromatography on a chiral AGP column

A direct HPLC method was developed for the determination of benazepril, its stereoisomers and its active metabolite benazeprilat. The separation was achieved, without any derivatization, on an alpha(1)-acid glycoprotein-based column (chiral AGP). The influence of pH, temperature, buffer ionic strength and organic modifiers of the mobile phase on retention and enantioselectivity was evaluated. The optimised method permits baseline separation of benazepril, its stereoisomers and its metabolite in less than 35 min, without the use of charged organic modifiers or tandem coupled chromatography. (C) 1998 Elsevier Science B.V. All rights reserved.