Absence of mutations in marRAB or soxRS in acrB-overexpressing fluoroquinolone-resistant clinical and veterinary isolates of Escherichia coli

被引：92

作者：

Webber, MA ^{[1
]}

Piddock, LJV ^{[1
]}

机构：

[1] Univ Birmingham, Div Immun & Infect, Antimcirobial Agents Res Grp, Birmingham B15 2TT, W Midlands, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 05期

关键词：

D O I：

10.1128/AAC.45.5.1550-1552.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The amount of acrB, marA, and soxS mRNA was determined in 36 fluoroquinolone-resistant E, coli from humans and animals, 27 of which displayed a multiple-resistance phenotype. acrB mRNA was elevated in 11 of 36 strains. A mutation at codon 45 (Arg-->Cys) in acrR was found in 6 of these 11 strains. Ten of the 36 isolates appeared to overexpress soxS, and five appeared to overexpress marA. A number of mutations were found in the marR and soxR repressor genes, correlating with greater amounts of marA and soxS mRNA, respectively.

引用

收藏

页码：1550 / 1552

页数：3

相关论文

共 21 条

[1] Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon [J].

Alekshun, MN ;

Levy, SB .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (10) :2067-2075

[2] MOLECULAR CHARACTERIZATION OF THE SOXRS GENES OF ESCHERICHIA-COLI - 2 GENES CONTROL A SUPEROXIDE STRESS REGULON [J].

AMABILECUEVAS, CF ;

DEMPLE, B .

NUCLEIC ACIDS RESEARCH, 1991, 19 (16) :4479-4484

[3] ACTIVATION OF MULTIPLE ANTIBIOTIC-RESISTANCE AND BINDING OF STRESS-INDUCIBLE PROMOTERS BY ESCHERICHIA-COLI ROB PROTEIN [J].

ARIZA, RR ;

LI, ZY ;

RINGSTAD, N ;

DEMPLE, B .

JOURNAL OF BACTERIOLOGY, 1995, 177 (07) :1655-1661

[4] Differential expression of over 60 chromosomal genes in Escherichia coli by constitutive expression of MarA [J].

Barbosa, TM ;

Levy, SB .

JOURNAL OF BACTERIOLOGY, 2000, 182 (12) :3467-3474

[5] Defining a rob regulon in Escherichia coli by using transposon mutagenesis [J].

Bennik, MHJ ;

Pomposiello, PJ ;

Thorne, DF ;

Demple, B .

JOURNAL OF BACTERIOLOGY, 2000, 182 (13) :3794-3801

[6] Contributions of individual mechanisms to fluoroquinolone resistance in 36 Escherichia coli strains isolated from humans and animals [J].

Everett, MJ ;

Jin, YF ;

Ricci, V ;

Piddock, LJV .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (10) :2380-2386

[7] Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli [J].

Fralick, JA .

JOURNAL OF BACTERIOLOGY, 1996, 178 (19) :5803-5805

[8]

GEORGE AM, 1983, J BACTERIOL, V178, P2507

[9] The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals [J].

Ma, D ;

Alberti, M ;

Lynch, C ;

Nikaido, H ;

Hearst, JE .

MOLECULAR MICROBIOLOGY, 1996, 19 (01) :101-112

[10] MOLECULAR-CLONING AND CHARACTERIZATION OF ACRA AND ACRE GENES OF ESCHERICHIA-COLI [J].

MA, D ;

COOK, DN ;

ALBERTI, M ;

PON, NG ;

NIKAIDO, H ;

HEARST, JE .

JOURNAL OF BACTERIOLOGY, 1993, 175 (19) :6299-6313