Surface plasmon resonance sensor for pesticide detection

被引:30
作者
Chegel, VI
Shirshov, YM
Piletskaya, EV
Piletsky, SA
机构
[1] Ukrainian Acad Sci, Inst Semicond Phys, UA-252028 Kiev, Ukraine
[2] Ukrainian Acad Sci, Inst Mol Biol & Genet, UA-252650 Kiev, Ukraine
关键词
surface plasmon resonance; atrazine; plastoquinone;
D O I
10.1016/S0925-4005(98)00084-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The optoelectronic biosensor, based on surface plasmon resonance (SPR) for detection of photosynthesis-inhibiting pesticides in aqueous solutions was presented. The pesticide capability to replace plastoquinone from its complex with D1 protein was used for the detection. This replacement reaction results in the changes of the optical characteristics of protein layer, immobilized on the gold surface. Monitoring of these changes with SPR-technique permits to determine 0.05-5.0 mu g/ml pesticide in solution within 1 h. (C) 1998 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:456 / 460
页数:5
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