Study of biochemical reactions in thin organic films by means of evanescent optical wave

被引：6

作者：

Ksenevich, TI

Beloglazov, AA

Nikitin, PI

Kalabina, NA

Zaitsev, SY

机构：

[1] RUSSIAN ACAD SCI, INST GEN PHYS, MOSCOW 117942, RUSSIA

[2] RUSSIAN ACAD SCI, SHEMYAKIN INST BIOORGAN CHEM, MOSCOW 117871, RUSSIA

来源：

APPLIED SURFACE SCIENCE | 1996年 / 92卷

关键词：

D O I：

10.1016/0169-4332(95)00270-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

An optical biosensor has been developed for detection of pesticides, based on the evanescent optical wave technique. The concentration of the pesticide was measured in liquid. We specially originated a core complex of photosystem II film on a cheap disposable element. A setup on the basis of the Kretschmann arrangement was improved by using a computer-controlled angular scanning system. The detection concentration limits were 10(-11) M for dinitrophenole and 10(-10) M for paraoxon. The results obtained show the perspective of the integral method for detection of different kinds of pesticides at extremely low concentration levels of 0.1 ppb.

引用

页码：426 / 430

页数：5

共 14 条

[1]

ANDERSSON B., 1991, CURR TOP BIOENERG, V16, P1

[2] INTEGRATED OPTICAL IMMUNOSENSOR FOR S-TRIAZINE DETERMINATION - REGENERATION, CALIBRATION AND LIMITATIONS [J].