Environmental air sampling to detect Exotic Newcastle Disease Virus in two California commercial poultry flocks

被引:24
作者
Hietala, SK
Hullinger, PJ
Crossley, BM
Kinde, H
Ardans, AA
机构
[1] Univ Calif Davis, California Anim Hlth & Food Safety Lab, Davis, CA 95616 USA
[2] Calif Dept Food & Agr, Sacramento, CA 95814 USA
关键词
air sampler; environmental sampling; Exotic Newcastle Disease Virus; RT-PCR;
D O I
10.1177/104063870501700219
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The 2002-2003 Exotic Newcastle Disease (END) outbreak in Southern California poultry provided an opportunity to evaluate environmental air sampling as an efficient and cost-effective means of sampling flocks for detection of a circulating virus. Exotic Newcastle Disease virus was detected by real-time reverse transcriptase PCR from air samples collected using a wetted-wall cyclone-style air sampler placed within 2 m of birds in 2 commercial flocks suspected of being naturally exposed to END virus during the outbreak. Exotic Newcastle Disease virus was detected after 2 hours of air sampling the poultry-house environments of the 2 naturally infected flocks.
引用
收藏
页码:198 / 200
页数:3
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