Detection and differentiation of pathogenicity of avian paramyxovirus serotype 1 from field cases using one-step reverse transcriptase-polymerase chain reaction

被引：61

作者：

Creelan, JL ^{[1
]}

Graham, DA ^{[1
]}

McCullough, SJ ^{[1
]}

机构：

[1] Dept Agr & Rural Dev, Vet Sci Div, Belfast BT4 3SD, Antrim, North Ireland

来源：

AVIAN PATHOLOGY | 2002年 / 31卷 / 05期

关键词：

D O I：

10.1080/0307945021000005860

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Amplification of avian paramyxovirus serotype 1 (APMV-1)-specific nucleic acid fragments, followed by restriction endonuclease analysis (REA) using BglI, was carried out to type strains according to their virulence. Primer sequences were used to amplify a 202 base pair fragment, encompassing the fusion protein cleavage site, in a one-step reverse transcriptase-polymerase chain reaction (RT-PCR) test for detection of a range of field cases and reference strains of APMV-1. Subsequent REA of the amplified fragments enabled differentiation of low virulent lentogenic field and vaccine strains from more virulent mesogenic and velogenic field strains of APMV-1, including pigeon PMV-1. In the present paper, we report the development and application of a one-step RT-PCR test coupled with REA as a fast, specific method for both the detection and typing of APMV-1 from field samples.

引用

页码：493 / 499

页数：7

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