Detection and differentiation of pathogenicity of avian paramyxovirus serotype 1 from field cases using one-step reverse transcriptase-polymerase chain reaction

被引：61

作者：

Creelan, JL ^{[1
]}

Graham, DA ^{[1
]}

McCullough, SJ ^{[1
]}

机构：

[1] Dept Agr & Rural Dev, Vet Sci Div, Belfast BT4 3SD, Antrim, North Ireland

来源：

AVIAN PATHOLOGY | 2002年 / 31卷 / 05期

关键词：

D O I：

10.1080/0307945021000005860

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Amplification of avian paramyxovirus serotype 1 (APMV-1)-specific nucleic acid fragments, followed by restriction endonuclease analysis (REA) using BglI, was carried out to type strains according to their virulence. Primer sequences were used to amplify a 202 base pair fragment, encompassing the fusion protein cleavage site, in a one-step reverse transcriptase-polymerase chain reaction (RT-PCR) test for detection of a range of field cases and reference strains of APMV-1. Subsequent REA of the amplified fragments enabled differentiation of low virulent lentogenic field and vaccine strains from more virulent mesogenic and velogenic field strains of APMV-1, including pigeon PMV-1. In the present paper, we report the development and application of a one-step RT-PCR test coupled with REA as a fast, specific method for both the detection and typing of APMV-1 from field samples.

引用

页码：493 / 499

页数：7

共 32 条

[21] Newcastle disease: outbreak losses and control policy costs [J].

Leslie, J .

VETERINARY RECORD, 2000, 146 (21) :603-606

[22] Newcastle disease outbreaks in recent years in Western Europe were caused by an old (VI) and a novel genotype (VII) [J].

Lomniczi, B ;

Wehmann, E ;

Herczeg, J ;

Ballagi-Pordany, A ;

Kaleta, EF ;

Werner, O ;

Meulemans, G ;

Jorgensen, PH ;

Mante, AP ;

Gielkens, ALJ ;

Capua, I ;

Damoser, J .

ARCHIVES OF VIROLOGY, 1998, 143 (01) :49-64

[23]

MEULEMANS G, 1999, P JOINT 6 ANN M NAT, P34

[24] NUCLEOTIDE-SEQUENCE OF THE FUSION AND HEMAGGLUTININ NEURAMINIDASE GLYCOPROTEIN GENES OF NEWCASTLE-DISEASE VIRUS, STRAIN ULSTER - MOLECULAR-BASIS FOR VARIATIONS IN PATHOGENICITY BETWEEN STRAINS [J].

MILLAR, NS ;

CHAMBERS, P ;

EMMERSON, PT .

JOURNAL OF GENERAL VIROLOGY, 1988, 69 :613-620

[25] Pathotyping of Newcastle disease viruses by RT-PCR and restriction enzyme analysis [J].

Nanthakumar, T ;

Kataria, RS ;

Tiwari, AK ;

Butchaiah, G ;

Kataria, JM .

VETERINARY RESEARCH COMMUNICATIONS, 2000, 24 (04) :275-286

[26] Newcastle disease virus: detection and characterization by PCR of recent German isolates differing in pathogenicity [J].

Oberdorfer, A ;

Werner, O .

AVIAN PATHOLOGY, 1998, 27 (03) :237-243

[27] CHARACTERIZATION OF NEWCASTLE-DISEASE VIRUS ISOLATES BY REVERSE TRANSCRIPTION PCR COUPLED TO DIRECT NUCLEOTIDE SEQUENCING AND DEVELOPMENT OF SEQUENCE DATABASE FOR PATHOTYPE PREDICTION AND MOLECULAR EPIDEMIOLOGIC ANALYSIS [J].

SEAL, BS ;

KING, DJ ;

BENNETT, JD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (10) :2624-2630

[28] DETECTION OF NEWCASTLE-DISEASE VIRUS IN POULTRY VACCINES USING THE POLYMERASE CHAIN-REACTION AND DIRECT SEQUENCING OF AMPLIFIED CDNA [J].

STAUBER, N ;

BRECHTBUHL, K ;

BRUCKNER, L ;

HOFMANN, MA .

VACCINE, 1995, 13 (04) :360-364

[29] CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE [J].

THOMPSON, JD ;

HIGGINS, DG ;

GIBSON, TJ .

NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4673-4680

[30]

Thrusfield M., 1986, VET EPIDEMIOLOGY

← 1 2 3 4 →