DAPI fluorescence in nuclei isolated from tumors

被引：21

作者：

Krishan, A ^{[1
]}

Dandekar, PD ^{[1
]}

机构：

[1] Univ Miami, Sch Med, Div Expt Therapeut R 71, Radiat Oncol Dept,Sylvester Canc Ctr, Miami, FL 33136 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2005年 / 53卷 / 08期

关键词：

tumors; nuclear volume; flow cytometry; DAPl; DNA content;

D O I：

10.1369/jhc.4B6563.2005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In DNA histograms of some human solid tumors stained with nuclear isolation medium-4,6-diamidino-2-phenylindole dihydrochloride (NIM-DAPI), the coefficient of variation (CV) of the G(0)/G(1) peak was broad, and in nuclear volume vs DNA scattergrams, a prominent slope was seen. To determine the cause for this, nuclei from frozen breast tumors were stained with NIM-DAPI and analyzed after dilution or resuspension in PBS. In two-color (blue vs red) analysis, most of the slope and broad CV was due to red fluorescence of nuclei stained with NIM-DAPI, which was reduced on dilution or resuspension in PBS, resulting in elimination of the slope and tightening of the CV.

引用

页码：1033 / 1036

页数：4