Ionization enhancement in atmospheric pressure chemical ionization and suppression in electrospray ionization between target drugs and stable-isotope-labeled internal standards in quantitative liquid chromatography/tandem mass spectrometry

被引：206

作者：

Liang, HR ^{[1
]}

Foltz, RL ^{[1
]}

Meng, M ^{[1
]}

Bennett, P ^{[1
]}

机构：

[1] Div NWT Inc, Tandem Labs, Salt Lake City, UT 84124 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2003年 / 17卷 / 24期

关键词：

D O I：

10.1002/rcm.1268

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The phenomena of ionization suppression in electrospray ionization (ESI) and enhancement in atmospheric pressure chemical ionization (APCI) were investigated in selected-ion monitoring and selected-reaction monitoring modes for nine drugs and their corresponding stable-isotope-labeled internal standards (IS). The results showed that all investigated target drugs and their co-eluting isotope-labeled IS suppress each other's ionization responses in ESI. The factors affecting the extent of suppression in ESI were investigated, including structures and concentrations of drugs, matrix effects, and flow rate. In contrast to the ESI results, APCI caused seven of the nine investigated target drugs and their co-eluting isotope-labeled IS to enhance each other's ionization responses. The mutual ionization suppression or enhancement between drugs and their isotope-labeled IS could possibly influence assay sensitivity, reproducibility, accuracy and linearity in quantitative liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS). However, calibration curves were linear if an appropriate IS concentration was selected for a desired calibration range to keep the response factors constant. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：2815 / 2821

页数：7

共 20 条

[1] THE MATRIX EFFECT IN PARTICLE BEAM LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND RELIABLE QUANTIFICATION BY ISOTOPE-DILUTION [J].

BROWN, FR ;

DRAPER, WM .

BIOLOGICAL MASS SPECTROMETRY, 1991, 20 (09) :515-521

[2] Effect of affinity for droplet surfaces on the fraction of analyte molecules charged during electrospray droplet fission [J].

Cech, NB ;

Enke, CG .

ANALYTICAL CHEMISTRY, 2001, 73 (19) :4632-4639

[3]

Chang WT, 2002, J FORENSIC SCI, V47, P873

[4] Quantitative measurement of dihydrouridine in RNA using isotope dilution liquid chromatography mass spectrometry (LC/MS) [J].

Dalluge, JJ ;

Hashizume, T ;

McCloskey, JA .

NUCLEIC ACIDS RESEARCH, 1996, 24 (16) :3242-3245

[5] RAPID-DETERMINATION OF METHANDROSTENOLONE IN EQUINE URINE BY ISOTOPE-DILUTION LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY [J].

EDLUND, PO ;

BOWERS, L ;

HENION, J ;

COVEY, TR .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 497 :49-57

[6]

ELLERBE P, 1989, ANAL CHEM, V61, P1710

[7] A predictive model for matrix and analyte effects in electrospray ionization of singly-charged ionic analytes [J].

Enke, CG .

ANALYTICAL CHEMISTRY, 1997, 69 (23) :4885-4893

[8]

HOUT MWJ, 2003, RAPID COMMUN MASS SP, V17, P245

[9] Determination of 4-hydroxy-3-methoxyphenylethylene glycol 4-sulfate in human urine using liquid chromatography-tandem mass spectrometry [J].

Jacob, P ;

Wilson, M ;

Yu, L ;

Mendelson, J ;

Jones, RT .

ANALYTICAL CHEMISTRY, 2002, 74 (20) :5290-5296

[10] Mass spectrometric assays for the tandem lesion 8,5′-cyclo-2′-deoxyguanosine in mammalian DNA [J].

Jaruga, P ;

Birincioglu, M ;

Rodriguez, H ;

Dizdaroglu, M .

BIOCHEMISTRY, 2002, 41 (11) :3703-3711

← 1 2 →