High-performance liquid chromatography intercomparative study for amino acid analysis in two tissues by PITC- and OPA-derivatizations

被引:9
作者
Avino, P
Campanella, L
Russo, MV
机构
[1] Univ Rome La Sapienza, Dipartimento Chim, I-00185 Rome, Italy
[2] Univ Molise, Fac Agr, DiSTAAM, I-86100 Campobasso, CB, Italy
关键词
amino acids; comparison; cysteine; phenylisothiocyanate; o-phthaldialdehyde;
D O I
10.1081/AL-100103598
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this paper, we have extensively investigated the pre-column derivatization method with o-phthaldialdehyde (OPA) to analyze cysteine, cystine, among with other 18 amino acids, using reversed-phase high-performance liquid chromatography analysis (RP-HPLC), and after we have compared it with an other method involving phenylisothiocyanate (PITC) in order to have a two sensitive methods to use accordingly the matrix. We have studied the analytical conditions for the OPA-reaction together with precision, linearity range, correlation coefficient (>0.998) and limit of detection (between 14 fmol and 1 pmol) for each amino acid. Then, we have applied the two methods to the analysis of larynx and kidney tissues: between the two methods, the amino acid recoveries (between 80% and 100%) and the correspondence of the OPA and PTC results are satisfactory considering the different involved derivatization procedures. PTC- and OPA-chromatograms obtained in the analysis of actual samples are reported together with the amino acid level, with particular regard to the cysteine/cystine determination.
引用
收藏
页码:867 / 882
页数:16
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