Intravital Imaging

被引：384

作者：

Pittet, Mikael J. ^{[1
,2
]}

Weissleder, Ralph ^{[1
,2
,3
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA

[2] Harvard Univ, Sch Med, Boston, MA 02114 USA

[3] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

来源：

CELL | 2011年 / 147卷 / 05期

基金：

美国国家卫生研究院;

关键词：

IN-VIVO; DENDRITIC CELLS; LYMPH-NODE; T-CELLS; FLUORESCENT PROTEINS; 2-PHOTON MICROSCOPY; HEMATOPOIETIC STEM; SINGLE CELLS; LIVING CELLS; BONE-MARROW;

D O I：

10.1016/j.cell.2011.11.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Until recently, the idea of observing life deep within the tissues of a living mouse, at a resolution sufficient to pick out cellular behaviors and molecular signals underlying them, remained a much-coveted dream. Now, a new era of intravital fluorescence microscopy has dawned. In this Primer, we review the technologies that made this revolution possible and demonstrate how intravital imaging is beginning to provide quantitative and dynamic insights into cell biology, immunology, tumor biology, and neurobiology.

引用

页码：983 / 991

页数：9

共 75 条

[1] Quantitative analysis of pathways controlling extrinsic apoptosis in single cells [J].

Albeck, John G. ;

Burke, John M. ;

Aldridge, Bree B. ;

Zhang, Mingsheng ;

Lauffenburger, Douglas A. ;

Sorger, Peter K. .

MOLECULAR CELL, 2008, 30 (01) :11-25

[2] Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model [J].

Alexander, Stephanie ;

Koehl, Gudrun E. ;

Hirschberg, Markus ;

Geissler, Edward K. ;

Friedl, Peter .

HISTOCHEMISTRY AND CELL BIOLOGY, 2008, 130 (06) :1147-1154

[3] Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging [J].

Andresen, Volker ;

Alexander, Stephanie ;

Heupel, Wolfgang-Moritz ;

Hirschberg, Markus ;

Hoffman, Robert M. ;

Friedl, Peter .

CURRENT OPINION IN BIOTECHNOLOGY, 2009, 20 (01) :54-62

[4]

[Anonymous], 1839, ERLAUTERUNGSTAFELN P

[5] Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior [J].

Auffray, Cedric ;

Fogg, Darin ;

Garfa, Meriem ;

Elain, Gaelle ;

Join-Lambert, Olivier ;

Kayal, Samer ;

Sarnacki, Sabine ;

Cumano, Ana ;

Lauvau, Gregoire ;

Geissmann, Frederic .

SCIENCE, 2007, 317 (5838) :666-670

[6] Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy [J].

Barretto, Robert P. J. ;

Ko, Tony H. ;

Jung, Juergen C. ;

Wang, Tammy J. ;

Capps, George ;

Waters, Allison C. ;

Ziv, Yaniv ;

Attardo, Alessio ;

Recht, Lawrence ;

Schnitzer, Mark J. .

NATURE MEDICINE, 2011, 17 (02) :223-U120

[7] Visualizing the Functional Diversification of CD8+ T Cell Responses in Lymph Nodes [J].

Beuneu, Helene ;

Lemaitre, Fabrice ;

Deguine, Jacques ;

Moreau, Helene D. ;

Bouvier, Isabelle ;

Garcia, Zacarias ;

Albert, Matthew L. ;

Bousso, Philippe .

IMMUNITY, 2010, 33 (03) :412-423

[8] In vivo imaging of cytotoxic T cell infiltration and elimination of a solid tumor [J].

Boissonnas, Alexandre ;

Fetler, Luc ;

Zeelenberg, Ingrid S. ;

Hugues, Stphanie ;

Amigorena, Sebastian .

JOURNAL OF EXPERIMENTAL MEDICINE, 2007, 204 (02) :345-356

[9] T-cell activation by dendritic cells in the lymph node: lessons from the movies [J].

Bousso, Philippe .

NATURE REVIEWS IMMUNOLOGY, 2008, 8 (09) :675-684

[10] Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells [J].

Boutrus, Steven ;

Greiner, Cherry ;

Hwu, Derrick ;

Chan, Michael ;

Kuperwasser, Charlotte ;

Lin, Charles P. ;

Georgakoudi, Irene .

JOURNAL OF BIOMEDICAL OPTICS, 2007, 12 (02)

← 1 2 3 4 5 6 7 8 →