Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging

被引：154

作者：

Andresen, Volker ^{[1
,2
,3
]}

Alexander, Stephanie ^{[1
,2
]}

Heupel, Wolfgang-Moritz ^{[1
,2
]}

Hirschberg, Markus ^{[1
,2
]}

Hoffman, Robert M. ^{[4
,5
]}

Friedl, Peter ^{[1
,2
,6
]}

机构：

[1] Univ Wurzburg, Rudolf Virchow Ctr Expt Biomed, D-97080 Wurzburg, Germany

[2] Univ Wurzburg, Dept Dermatol Venerol & Allergol, D-97080 Wurzburg, Germany

[3] LaVis BioTec GmbH, D-33607 Bielefeld, Germany

[4] AntiCancer Inc, San Diego, CA 92111 USA

[5] Univ Calif San Diego, Dept Surg, San Diego, CA 92103 USA

[6] Radboud Univ Nijmegen, Med Ctr, Nijmegen Ctr Mol Life Sci, Microscop Imaging Ctr, NL-6500 HB Nijmegen, Netherlands

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2009年 / 20卷 / 01期

关键词：

IN-VIVO; FLUORESCENCE MICROSCOPY; CELLS;

D O I：

10.1016/j.copbio.2009.02.008

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Multiphoton microscopy (MPM) is the method of choice for investigating cells and cellular functions in deep tissue sections and organs. Here we present the setup and applications of infrared-(IR-)MPM using excitation wavelengths above 1080 nm. IR-MPM enables the use of red fluorophores and fluorescent proteins, doubles imaging depth, improves second harmonic generation of tissue structures, and strongly reduces phototoxicity and photobleaching, compared with conventional MPM. Furthermore, it still provides subcellular resolution at depths of several hundred micrometers and thus will enhance long-term live cell and deep tissue microscopy.

引用

页码：54 / 62

页数：9

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