Effects of carbon source and metabolic engineering on butyrate production in Escherichia coli

被引：12

作者：

Joung, Sea-Mi ^{[1
]}

Kurumbang, Nagendra Prasad ^{[1
]}

Sang, Byoung-In ^{[2
]}

Oh, Min-Kyu ^{[1
]}

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136713, South Korea

[2] Korea Inst Sci & Technol, Clean Energy Ctr, Seoul 136791, South Korea

来源：

KOREAN JOURNAL OF CHEMICAL ENGINEERING | 2011年 / 28卷 / 07期

关键词：

Escherichia coli; Clostridium acetobutylicum; Butyrate; Metabolic Engineering; CLOSTRIDIUM-ACETOBUTYLICUM; EXTRACTIVE FERMENTATION; ACID PRODUCTION; MAJOR GLUCOSE; GENES; PTSG; EXPRESSION; PATHWAYS; ACETATE; TYROBUTYRICUM;

D O I：

10.1007/s11814-011-0032-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Butyrate was produced in recombinant Escherichia coli strains by applying metabolic engineering strategies. The genes for producing butyrate were cloned from Clostridium acetobutylicum and then expressed in E. coli. To study important factors for improving the productivity of butyrate, we deleted pta and ptsG genes in E. coli and compared the effects of these gene deletions in E. coli B and K strains. The effect of carbon sources, glucose and glycerol, was also compared. A significant improvement of butyrate production was made when glycerol was used as a carbon source, resulting in 0.56 g/l of butyrate in LB medium with 1% (v/v) glycerol.

引用

页码：1587 / 1592

页数：6

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