Nucleic acid cleavage by a Cu(II) polyaza macrocyclic complex

被引：38

作者：

Oliveira, MCB

Couto, MSR

Severino, PC

Foppa, T

Martins, GTS

Szpoganicz, B

Peralta, RA

Neves, A

Terenzi, H ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Dept Bioquim, Lab Expressao Gen, CCB, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Santa Catarina, Dept Quim, Lab Equilibrio Quim, BR-88040900 Florianopolis, SC, Brazil

[3] Univ Fed Santa Catarina, Dept Quim, Lab Bioinorgan & Cristalog, BR-88040900 Florianopolis, SC, Brazil

来源：

POLYHEDRON | 2005年 / 24卷 / 04期

关键词：

DNA cleavage; polyaza ligand; binuclear copper complex; BMXD; chemical nuclease; DNA-drug interaction;

D O I：

10.1016/j.poly.2004.12.016

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The search for efficient artificial nucleases is a consequence of the huge efforts towards DNA sequencing by genome projects around the world. In the present study, we describe the activity of the Cu(II)-BMXD complex (BMXD = 3,6,9,17,20,23-hexaazatri-cyclo[23.11.1(11,15)]triaconta-1(29),11(30),12,14,25,27-hexaene) as a DNA cleavage agent. The rate constant for the DNA backbone cleavage reaction at room temperature (25 degrees C) and pH 7.0 was estimated to be 1.1 x 10(-6) s(-1), which corresponds, approximately, to a half-life (t(1/2)) of one week. We demonstrated that the DNA cleavage reaction promoted by the Cu2BMXD complex may occur via an oxidative pathway through performing reactions,in the presence of free-radical acceptors and comparing the activity under aerobic and anaerobic conditions. The Cu2BMXD complex reported here is established as a novel artificial nuclease. (c) 2005 Elsevier.Ltd. All rights reserved.

引用

页码：495 / 499

页数：5

共 46 条

[1] DNA cleavage by Fe(II)•bleomycin conjugated to a solid support [J].