Purification and characterization of an enzyme involved in biochemical transformation of arteannuin B to artemisinin from Artemisia annua

被引：23

作者：

Dhingra, V ^{[1
]}

Narasu, ML ^{[1
]}

机构：

[1] Jawaharlal Nehru Technol Univ, Inst Post Grad Studies & Res, Ctr Biotechnol, Hyderabad 500028, Andhra Pradesh, India

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 281卷 / 02期

关键词：

artemisinin; arteannuin B; malaria; Artemisia annua; bioconversion; purification;

D O I：

10.1006/bbrc.2000.4197

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein involved in the conversion of arteannuin B to artemisinin has been purified from the leaves of Artemisia annua, The pure protein found to be homogenous on Native gel electrophoresis showed two major bands of 21 and 11 kDa on 12% SDS-PAGE. Molecular weight estimation of native protein indicated an apparent molecular mass of 66,000 Daltons, This protein is able to achieve 58% conversion. It has a K-m of 0.5 mM for arteannuin B and a pH optima between 7.0-7.2. It is maximally active at 30 degreesC. (C) 2001 Academic Press.

引用

页码：558 / 561

页数：4

共 9 条

[1]

Delabays N., 1993, Acta Horticulturae, P203

[2] Partial purification of proteins involved in the bioconversion of arteannuin B to artemisinin [J].

Dhingra, V ;

Rajoli, C ;

Narasu, ML .

BIORESOURCE TECHNOLOGY, 2000, 73 (03) :279-282

[3] Artemisinin: present status and perspectives [J].

Dhingra, V ;

Rao, KV ;

Narasu, ML .

BIOCHEMICAL EDUCATION, 1999, 27 (02) :105-109

[4]

Dhingra V, 2000, CURR SCI INDIA, V78, P709

[5]

Dhingra V, 1999, LIFE SCI, V66, P279, DOI 10.1016/S0024-3205(99)00356-2

[6]

Gupta M. M., 1997, Journal of Medicinal and Aromatic Plant Sciences, V19, P968

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8] BIOCONVERSION OF ARTEANNUIN-B TO ARTEMISININ [J].

NAIR, MSR ;

BASILE, DV .

JOURNAL OF NATURAL PRODUCTS, 1993, 56 (09) :1559-1566

[9]

Scopes R.K., 1994, PROTEIN PURIFICATION, P71

← 1 →