共 52 条
Characterization of Arabidopsis genes involved in biosynthesis of polyamines in abiotic stress responses and developmental stages
被引:168
作者:
Urano, K
Yoshiba, Y
Nanjo, T
Igarashi, Y
Seki, M
Sekiguchi, F
Yamaguchi-Shinozaki, K
Shinozaki, K
机构:
[1] RIKEN, Tsukuba Inst, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan
[2] Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 305, Japan
[3] Hitachi Ltd, Cent Res Lab, Life Sci Res Ctr, Hatoyama, Saitama, Japan
[4] Hitachi Ltd, Dept Res & Dev, Life Sci Grp, Kawagoe, Saitama 3501165, Japan
[5] Japan Womens Univ, Grad Sch Sci, Div Mat & Biol Funct Sci, Bunkyo Ku, Tokyo 112, Japan
[6] Minist Agr Forestry & Fisheries, Biol Resources Div, JIRCAS, Tsukuba, Ibaraki, Japan
关键词:
Arabidopsis thaliana;
environmental stress;
polyamines;
sexual organ-specific expression;
D O I:
10.1046/j.1365-3040.2003.01108.x
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
To characterize the genes for enzymes involved in the biosynthesis of polyamines (PAs), their expression profiles were investigated and the levels of PAs in Arabidopsis thaliana quantified. In the Arabidopsis genome, eight genes involved in PAs biosynthesis were identified and the expression profiles of these genes were analysed, not only under abiotic stress to determine whether they were stress-inducible, constitutive, or stress-repressible, but also in various organs to show their tissue specificity. AtADC2 and AtSPMS mRNAs, encoding arginine decarboxylase and spermine synthase, clearly increased in response to NaCl and dehydration and abscisic acid treatments. Stress-inducible accumulation of AtADC2 mRNA correlated with putrescine (Put) accumulation under NaCl and dehydration treatments. In a cold condition, AtSAMDC2 mRNA increased significantly. AtADC2 and AtSAMDC2 mRNA were expressed in sexual organs such as flowers, buds and immature siliques. PAs also accumulated in sexual organs. These results suggest that the transcripts of eight genes involved in PA biosynthesis show different profiles of expression not only in response to environmental stress but also during plant development.
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页码:1917 / 1926
页数:10
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