Selective separation of amino acids by reactive extraction

被引：64

作者：

Cascaval, D

Oniscu, C

Galaction, AI

机构：

[1] Tech Univ Iasi, Fac Ind Chem, Dept Organ Ind & Biotechnol, Iasi 6600, Romania

[2] Univ Med & Pharm Iasi, Fac Med Bioengn, Iasi 6600, Romania

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2001年 / 7卷 / 03期

关键词：

Amino acids; di-(2-ethylhexyl)phosphoric acid; reactive extraction; selective extraction;

D O I：

10.1016/S1369-703X(00)00096-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The method of reactive extraction with di-(2-ethylhexyl)phosphoric acid (D2EHPA) for the separation of a range of amino acids is studied. The results obtained on the individual reactive extraction indicated the possibility of the amino acids selective separation as a function of the pH value of aqueous solution and the acidic or basic character of each amino acid. Thus, using multistage extraction, the total separation of the following amino acids groups has been performed: neutral amino acids (L-glycine, L-alanine, L-tryptophan) at pH 5-5.5 (nine extraction stages), basic amino acids (L-lysine, L-arginine) and L-cysteine at pH 4-4.5 (ten extraction stages), L-histidine at pH 3-3.5 (five extraction stages), and acidic amino acids (L-aspartic acid, L-glutamic acid) at pH 2-2.5 (three extraction stages). (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：171 / 176

页数：6