Persistence of DNA in cell cultures may jeopardize the analysis of human herpesvirus 6 dynamics by means of real-time PCR

被引：9

作者：

Bonnafous, P

Gautheret-Dejean, A

Boutolleau, D

Caïola, D

Agut, H ^{[1
]}

机构：

[1] Grp Hosp Pitie Salpetriere, CERVI, UPRES EA238, Virol Lab, F-75013 Paris, France

[2] Fac Sci Pharmaceut & Biol Paris, Microbiol Lab, F-75006 Paris, France

[3] CHU Bicetre, Fac Med Paris Sud, Lab Bacteriol Virol, F-94275 Le Kremlin Bicetre, France

来源：

JOURNAL OF VIROLOGICAL METHODS | 2005年 / 125卷 / 01期

关键词：

virus cell load; virus growth kinetics; susceptibility assay; antiviral drug;

D O I：

10.1016/j.jviromet.2004.12.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of real-time PCR has been described previously for analysing both the replication kinetics and antiviral susceptibility of human herpesvirus 6 in MT4 cells. It is now reported that viral DNA persists in infected cell culture long after the end of lytic virus replication. Consequently, high levels of DNA may correspond to an absence of infectivity and late readout occurring after the exponential phase of virus growth may lead to misinterpretation of the results of susceptibility assays. These limitations must be borne in mind when using real-time PCR. (c) 2004 Elsevier B.V. All rights reserved.

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页码：95 / 98

页数：4

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