Production of microporous aluminum oxide electrodes as supports for tethered lipid bilayers of large surface area

被引:8
作者
Fliniaux, O [1 ]
Elie-Caille, C [1 ]
Pantigny, J [1 ]
Bourdillon, C [1 ]
机构
[1] Univ Technol Compiegne, CNRS, UMR 6022, F-60205 Compiegne, France
关键词
porous aluminum oxide; phospholipid bilayer; two-dimensional electrochemistry; ubiquinone lateral mobility;
D O I
10.1016/j.elecom.2005.04.023
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
A composite electrode made by association of gold and porous aluminum oxide has been used as template for a supported lipid bilayer. An oxide pore size of 170 nm was chosen so that lipid vesicles or proteoliposomes can enter in the pore and cover the pore wall. Triggered vesicle fusion produced a stable and continuous lipid bilayer supported on the large surface area of the honeycomb structure. Continuity of the supported bilayer was attested by electrochemical measurement of the lateral mobility of ubiquinone, a water-insoluble electroactive marker, dissolved at low concentration in the hydrophobic leaflet of the bilayer. (C) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:697 / 702
页数:6
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