A study of the interaction between a new reagent and serum albumin by fluorescence spectroscopy

被引：72

作者：

Cui, FL

Fan, J

Ma, DL

Liu, MC

Chen, XG

Hu, ZD ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

[2] Henan Normal Univ, Coll & Univ Henan Province, Sch Chem & Environm Sci, Key Lab Environm Sci & Engn, Xinxiang, Henan, Peoples R China

来源：

ANALYTICAL LETTERS | 2003年 / 36卷 / 10期

基金：

中国国家自然科学基金;

关键词：

N-n-hexyl-N '-(sodium p-aminobenzenesulfonate)thiourea (HXPT); bovine serum albumin (BSA); human serum albumin (HSA); fluorescence spectroscopy;

D O I：

10.1081/AL-120023708

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The synthesis of a new reagent, saturated fatty hydrocarbon substituting group compound N-n-hexyl-N'-(sodium p-aminobenzenesulfonate) thiourea (HXPT), is described. The interactions between HXPT and bovine serum albumin or human serum albumin were studied by fluorescence spectroscopy. The binding constants of HXPT with BSA or HSA were determined at different temperatures under the optimum conditions. The binding sites were obtained and the acting force were suggested to be mainly hydrophobic. The effect of common ions on the binding constants was also investigated. A practical method was proposed for the determination of HXPT in bovine serum or human serum samples.

引用

页码：2151 / 2166

页数：16

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