Hypophysotropic activity of histone H3 in vitro

被引：2

作者：

Brown, OA ^{[1
]}

Sosa, YE ^{[1
]}

Naumovich, D ^{[1
]}

Hereñú, CB ^{[1
]}

Goya, RG ^{[1
]}

机构：

[1] Natl Univ La Plata, Fac Med, INIBIOLP, RA-1900 La Plata, Argentina

来源：

PEPTIDES | 2003年 / 24卷 / 05期

关键词：

histones; hypophysotropic activity; pituitary cells; second messengers; apoptotic signals;

D O I：

10.1016/S0196-9781(03)00132-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To assess the effect of histone H3 on pituitary hormone secretion, rat anterior pituitary (AP) cells were used and growth hormone, prolactin, thyrotropin, luteinizing hormone and follicle stimulating hormone measured by radioimmunoassay. Incubation of cells with H3 (1, 6, and 30 muM) stimulated the release of all five hormones in a dose-dependent manner. This effect was blocked by preincubation of H3 with an anti-H3 antibody. Incubation of AP cells with 6 muM H3 in the presence of specific AP hormone secretagogues (GRP-6, thyrotropin-releasing hormone (TRH), gonadotropin-releasing hormone (GnRH)) showed additive effects on hormone secretion. Pharmacological experiments suggested that calcium- and diacylglycerol- (DAG) associated pathways, but not cAMP, participate in the hypophysiotropic activity of H3. Our results confirm previous evidence that histones may act as hypophysiotropic signals. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：671 / 678

页数：8

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