Gene manipulation in parasitic helminths

被引：32

作者：

Boyle, JP ^{[1
]}

Yoshino, TP ^{[1
]}

机构：

[1] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2003年 / 33卷 / 11期

关键词：

gene regulation; parasitic helminth; transformation; Transfection; RNA interference; functional genomics;

D O I：

10.1016/S0020-7519(03)00159-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In light of recent growth in available DNA sequence information for a number of parasitic helminths, it is crucial that suitable gene manipulation technologies are developed to facilitate functional genomic studies in these organisms. In this review we discuss recent progress in the development of these technologies in nematode and platyhelminth parasites of medical and veterinary importance. Specifically, the current status of transient transfection, double-stranded RNA interference and antisense RNA as viable techniques for the manipulation of parasitic helminth gene expression is presented. In addition, the potential for the development of stable, or germ-line, transformation methods in these organisms is also discussed. (C) 2003 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：1259 / 1268

页数：10

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