Evidence for an unfolding and refolding pathway in cytochrome c

被引：135

作者：

Xu, YJ ^{[1
]}

Mayne, L ^{[1
]}

Englander, SW ^{[1
]}

机构：

[1] Univ Penn, Dept Biochem & Biophys, Johnson Res Fdn, Philadelphia, PA 19104 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1998年 / 5卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/1810

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been suggested that three partially unfolded forms detected in a native state hydrogen exchange study of oxidized cytochrome c may represent sequential intermediates in an unfolding-refolding pathway. To better define the structure of each intermediate a 'stability labeling' method was used in which the stability of a given segment against unfolding was changed. The condition - folded or unfolded - of the stability-labeled segment in each intermediate could then be determined. The structures found are discrete and native-like and are just those necessary for a sequential pathway.

引用

页码：774 / 778

页数：5

共 39 条

[21] HVIDT AASE, 1966, ADVANCE PROTEIN CHEM, V21, P287, DOI 10.1016/S0065-3233(08)60129-1
[22] Hydrogen exchange in chymotrypsin inhibitor 2 probed by denaturants and temperature
Itzhaki, LS
Neira, JL
Fersht, AR
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 270 (01) : 89 - 98
[23] KIM PS, 1990, ANNU REV BIOCHEM, V59, P631, DOI 10.1146/annurev.biochem.59.1.631
[24] SPECIFIC INTERMEDIATES IN THE FOLDING REACTIONS OF SMALL PROTEINS AND THE MECHANISM OF PROTEIN FOLDING
KIM, PS
BALDWIN, RL
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 : 459 - 489
[25] MOLSCRIPT - A PROGRAM TO PRODUCE BOTH DETAILED AND SCHEMATIC PLOTS OF PROTEIN STRUCTURES
KRAULIS, PJ
[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 : 946 - 950
[26] RESIDUAL HELICAL STRUCTURE IN THE C-TERMINAL FRAGMENT OF CYTOCHROME-C
KURODA, Y
[J]. BIOCHEMISTRY, 1993, 32 (05) : 1219 - 1224
[27] PROTEIN FOLDING FUNNELS - A KINETIC APPROACH TO THE SEQUENCE STRUCTURE RELATIONSHIP
LEOPOLD, PE
MONTAL, M
ONUCHIC, JN
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (18) : 8721 - 8725
[28] Heme-peptide models for hemoproteins .1. Solution chemistry of N-acetylmicroperoxidase-8
Munro, OQ
Marques, HM
[J]. INORGANIC CHEMISTRY, 1996, 35 (13) : 3752 - 3767
[29] Hydrogen exchange in chymotrypsin inhibitor 2 probed by mutagenesis
Neira, JL
Itzhaki, LS
Otzen, DE
Davis, B
Fersht, AR
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 270 (01) : 99 - 110
[30] SOLUTION STRUCTURE OF HORSE HEART FERROCYTOCHROME-C DETERMINED BY HIGH-RESOLUTION NMR AND RESTRAINED SIMULATED ANNEALING
QI, PX
DISTEFANO, DL
WAND, AJ
[J]. BIOCHEMISTRY, 1994, 33 (21) : 6408 - 6417

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