Mycoplasmas regulate the expression of heat-shock protein genes through CIRCE-HrcA interactions

被引：16

作者：

Chang, Li-Jen ^{[1
]}

Chen, Wei-Hsiuan ^{[1
]}

Minion, F. Chris ^{[2
]}

Shiuan, David ^{[1
,3
]}

机构：

[1] Natl Dong Hwa Univ, Dept Life Sci, Inst Biotechnol, Hualien 974, Taiwan

[2] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA USA

[3] Natl Dong Hwa Univ, Bioinformat Program, Hualien 974, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 367卷 / 01期

关键词：

CIRCE element; heat-shock protein; HrcA repressor; Mycoplasma hyopneumoniae; negative regulation; electrophoretic mobility shift assay;

D O I：

10.1016/j.bbrc.2007.12.124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mycoplasmas in general are rarely exposed to severe environmental changes except during its colonization and infection processes. Genomic analysis indicates that Mycoplasma hyopneumoniae possesses the genes of a single sigma factor and the HrcA repressor of negative regulation of the heat-shock response. A perfect inverted repeat sequence (5'-CTGGCACTT-N-9-AAGTGCCAA-3') upstream of the DnaK gene has also been identified. In the present study, we demonstrate the functionality of HrcA-CIRCE interactions using the gel electrophoretic mobility shift assay. The presence of the unique sigma factor, HrcA repressor, and the CIRCE-like sequences reveals that mycoplasmal species may all use the negative regulatory mechanism in the heat-shock response. It is conceivable that mycoplasmas may have evolved a single HrcA repressor-based mechanism which might be the most simple and economical way of controlling HSP gene expression. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：213 / 218

页数：6

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