Control of a Nanoscopic-to-Macroscopic Transition: Modulated Phases in Four-Component DSPC/DOPC/POPC/Chol Giant Unilamellar Vesicles

被引：99

作者：

Konyakhina, Tatyana M. ^{[1
]}

Goh, Shih Lin ^{[1
]}

Amazon, Jonathan ^{[1
]}

Heberle, Frederick A. ^{[2
]}

Wu, Jing ^{[3
]}

Feigenson, Gerald W. ^{[1
]}

机构：

[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

[2] Oak Ridge Natl Lab, Neutron Scattering Sci Div, Oak Ridge, TN USA

[3] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

BIOPHYSICAL JOURNAL | 2011年 / 101卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

MODEL BIOMEMBRANES; MEMBRANES; DOMAINS;

D O I：

10.1016/j.bpj.2011.06.019

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have found modulated phase morphology in a particular region of composition within the liquid-ordered + liquid-disordered coexistence region in the four-component lipid bilayer mixture DSPC/DOPC/POPC/Chol. By controlling lipid composition, we could see distinct types of modulated liquid-liquid phase morphologies, including linear, irregular, and angular features in giant unilamellar vesicles. We used a combination of confocal, two-photon, wide-field fluorescence, and differential interference contrast microscopies, and used stringent controls to minimize light-induced artifacts. These studies establish that both the size and morphology of membrane rafts can be controlled by the concentration and the type of low-melting lipid in mixtures with cholesterol and a high-melting lipid.

引用

页码：L8 / L10

页数：3

共 14 条

[1] Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension [J].

Baumgart, T ;

Hess, ST ;

Webb, WW .

NATURE, 2003, 425 (6960) :821-824

[2] Formation and regulation of lipid microdomains in cell membranes: Theory, modeling, and speculation [J].

Fan, Jun ;

Sammalkorpi, Maria ;

Haataja, Mikko .

FEBS LETTERS, 2010, 584 (09) :1678-1684

[3] Comparison of Three Ternary Lipid Bilayer Mixtures: FRET and ESR Reveal Nanodomains [J].

Heberle, Frederick A. ;

Wu, Jing ;

Goh, Shih Lin ;

Petruzielo, Robin S. ;

Feigenson, Gerald W. .

BIOPHYSICAL JOURNAL, 2010, 99 (10) :3309-3318

[4] An introduction to critical points for biophysicists; observations of compositional heterogeneity in lipid membranes [J].

Honerkamp-Smith, Aurelia R. ;

Veatch, Sarah L. ;

Keller, Sarah L. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2009, 1788 (01) :53-63

[5] Bending-mediated superstructural organizations in phase-separated lipid membranes [J].

Kaizuka, Yoshihisa ;

Groves, Jay T. .

NEW JOURNAL OF PHYSICS, 2010, 12

[6] Lipid Rafts As a Membrane-Organizing Principle [J].

Lingwood, Daniel ;

Simons, Kai .

SCIENCE, 2010, 327 (5961) :46-50

[7] Phase segregation on different length scales in a model cell membrane system [J].

Liu, J ;

Qi, SA ;

Groves, JT ;

Chakraborty, AK .

JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (42) :19960-19969

[8] STRUCTURES AND TRANSITIONS IN LIPID MONOLAYERS AT THE AIR-WATER-INTERFACE [J].

MCCONNELL, HM .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1991, 42 :171-195

[9] Dynamic observations of nanoscale self-assembly on solid surfaces [J].

Plass, R ;

Bartelt, NC ;

Kellogg, GL .

JOURNAL OF PHYSICS-CONDENSED MATTER, 2002, 14 (16) :4227-4240

[10] Formation and spatio-temporal evolution of periodic structures in lipid bilayers [J].

Rozovsky, S ;

Kaizuka, Y ;

Groves, JT .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (01) :36-37

← 1 2 →